亲和短肽用于快速检测转基因蛋白Cry3Bb

发布时间：2018-03-22 13:02

  本文选题：CryBb毒素　切入点：转基因植物　出处：《江苏农业学报》2017年03期 　论文类型：期刊论文

【摘要】：本研究利用竞争洗脱法淘选与Cry3Bb毒素具有亲和力的特异性短肽,以此建立间接竞争酶联免疫吸附检测方法(IC-ELISA)。经过4轮淘选、富集,获得与靶蛋白具有较高亲和力的B10克隆(ACIHSPTALCGGG)。所建立的检测方法稳定性较好,变异系数在5%以内;线性检测范围为0.03~29.95μg/ml,线性回归方程为(Y=20.044x+50.407,R2=0.983 4),检测限为0.009 6μg/ml,在玉米样品中提取回收率为92.26%~101.22%。本研究所建立的ELISA检测方法为粮食中Cry3Bb蛋白的定量检测提供了有效的手段,在转基因产品的检验检疫中有较高的应用价值。
[Abstract]:In this study, an indirect competitive enzyme linked immunosorbent assay (IC-ELISAA) was established for the detection of short peptides with affinity to Cry3Bb toxin by competitive elution method. B10 clone with high affinity to target protein was obtained. The stability of the method was good, and the coefficient of variation was less than 5%. The linear detection range was 0.03 渭 g / ml, the linear regression equation was 20.044x 50.407R20.9834, the detection limit was 0.009 渭 g / ml, and the recovery rate was 92.26g / ml. The ELISA method established in this study provided an effective method for the quantitative detection of Cry3Bb protein in grain. It has high application value in the inspection and quarantine of transgenic products.
【作者单位】： 金陵科技学院园艺学院;江苏省农业科学院食品质量安全与检测研究所/农业部食品质量安全监控重点开放实验室;
【基金】：江苏省自然科学基金青年基金项目(BK20150114) 江苏省高校自然科学研究项目(14KJB 210004) 金陵科技学院博士启动项目(jit-6-201518);金陵科技学院校级科研基金孵化项目(C140501)
【分类号】：S188
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本文编号：1648761