油酸对奶牛乳腺上皮细胞内甘油三酯含量和乳脂肪合成相关基因表达的影响

发布时间：2018-03-22 15:23

本文选题：奶牛乳腺上皮细胞　切入点：油酸　出处：《中国畜牧杂志》2017年07期 　论文类型：期刊论文

【摘要】：本试验旨在探讨油酸对奶牛乳腺上皮细胞内甘油三酯含量和乳脂肪合成相关基因表达的影响。选取中国荷斯坦奶牛乳腺上皮细胞(BMECs)为试验材料,经纯化培养后,培养基中添加0(对照)、50、100、200、400μmol/L油酸,继续培养24 h。通过四甲基偶氮唑盐(MTT)比色法检测细胞活力;利用试剂盒检测胞内甘油三酯的含量;采用实时定量PCR法检测乳脂肪合成相关基因的表达。结果表明:油酸浓度为200、400μmol/L时,BMECs相对增殖率显著低于对照组与其他试验组(P0.05);添加100、200μmol/L的油酸促进了胞内甘油三酯的合成(P0.05);添加油酸上调了二酰甘油酰基转移酶(DGAT)基因表达量,50μmol/L的油酸显著上调了乙酰辅酶A羧化酶(ACC)和过氧化物酶体增殖物激活受体γ(PPARGγ)基因表达量(P0.05),100~400μmol/L的油酸显著抑制了硬脂酰辅酶A去饱和酶(SCD)、脂肪酸结合蛋白3(FABP3)、固醇调节原件结合蛋白1(SREBP-1)基因表达量。综上所述,50~100μmol/L的油酸对BMECs乳脂肪合成具有较好的促进效果。
[Abstract]:This experiment was conducted to investigate the effect of oleic acid on triglyceride content and milk fat synthesis related gene expression in bovine mammary epithelial cells. Selection of mammary epithelial cells China Holstein cows (BMECs) as the test material, purified culture, culture medium added with 0 (control), 50100200400 mol/L oleic acid, cultured for 24 h. by four methyl azo tetrazolium (MTT) cell vitality assay; detection of intracellular triglyceride content by ELISA kit; the expression was detected by quantitative real time PCR milk fat synthesis related genes. The results showed that oleic acid concentration is 200400 mol/L, the relative growth rate of BMECs was significantly lower than the control group and the other experimental groups (P0.05); add 100200 mol/L oleic acid promoted the synthesis of intracellular triglyceride (P0.05); adding oleic acid by two acyltransgerase (DGAT) gene expression, 50 mol/L oleic acid increased B Acyl coenzyme A carboxylase (ACC) and peroxisome proliferator activated receptor gamma (PPARG gamma) gene expression (P0.05), 100~400 mol/L oleic acid significantly inhibited the stearoyl coenzyme A desaturase (SCD), fatty acid binding protein 3 (FABP3), the original sterol regulatory binding protein 1 (SREBP-1) gene expression. In summary, 50~100 mol/L has a good effect on oleic acid BMECs milk fat synthesis.

【作者单位】：内蒙古农业大学动物科学学院;内蒙古自治区农牧业科学院动物营养研究所;
【基金】：现代农业(奶牛)产业技术体系建设专项资金(CAR S-37)
【分类号】：S823.5

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