水稻PHD-finger转录因子基因OsMsr16增强耐盐性的可能性研究

发布时间：2018-03-22 15:59

  本文选题：水稻　切入点：PHD-finger转录因子　出处：《基因组学与应用生物学》2016年07期 　论文类型：期刊论文

【摘要】：Os Msr16(Oryza sativa L.multi-stress-responsive gene 16)是一个新的水稻植物同源结构域(PHD)-finger家族转录因子基因。之前研究显示,Os Msr16受到低温、高温和干旱胁迫的诱导表达。本研究中,q RT-PCR分析表明Os Msr16同样受到盐胁迫的诱导表达,推测该基因可能参与植物在高盐胁迫下的生理调控。苗期盐处理条件下,与对照相比,过表达Os Msr16的转基因水稻植株叶片失绿面积较少,存活率更高。盐胁迫条件下,转基因植株中积累更高含量的脯氨酸和可溶性糖,而丙二醛含量和双氧水含量明显降低。转基因植株中超氧化物歧化酶(superoxide dismutase,SOD)活性和过氧化氢酶(catalase,CAT)活性明显高于对照。以上结果说明Os Msr16基因可能在植物防御盐胁迫过程中发挥重要作用。
[Abstract]:Os Msr16(Oryza sativa L.multi-stress-responsive gene 16 is a novel transcription factor gene of the PHD-finger family of rice plant homologous domains. Previous studies have shown that Os Msr16 is subjected to low temperature. In this study, RT-PCR analysis showed that Os Msr16 was also induced by salt stress, which suggested that the gene might be involved in physiological regulation of plants under high salt stress. Transgenic rice plants over-expressing Os Msr16 had less green area and higher survival rate. Under salt stress, higher content of proline and soluble sugar were accumulated in transgenic plants. The activities of superoxide dismutase (SOD) and catalase catalase (cat) in transgenic plants were significantly higher than those in the control. The above results suggested that Os Msr16 gene might protect plants from salt stress. Play an important role in the process.
【作者单位】： 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室;中国科学院研究生院;
【基金】：中国科学院“百人计划”项目(02200420062903) 国家自然科学基金项目(31171536)共同资助
【分类号】：Q943.2

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