敲除REG1同时过表达SNF1基因对工业面包酵母不加糖面团发酵的影响

发布时间：2018-03-22 23:34

本文选题：面包酵母　切入点：SNF　出处：《现代食品科技》2016年08期 　论文类型：期刊论文

【摘要】：研究了蛋白磷酸酶PP1调节亚基编码基因REG1缺失同时蛋白激酶基因SNF1过表达对工业面包酵母麦芽糖代谢和不加糖面团发酵的影响。比较分析REG1缺失同时SNF1过表达转化子BYKPS-R和亲本菌株BY14α、REG1缺失突变株BYK-R的MAL基因m RNA水平、麦芽糖酶和麦芽糖通透酶活力、麦芽糖代谢水平、不加糖面团发酵力,以及基本生长性能,结果表明,敲除REG1同时过表达SNF1能够显著提高麦芽糖代谢相关基因转录和酶活力,有效减弱葡萄糖阻遏,从而使面包酵母的麦芽糖消耗速率(葡萄糖被完全消耗完之前)较BY14α和BYK-R分别提高了18.59%和4.40%,不加糖面团发酵力分别提高了12.51%和3.22%。在REG1基因缺失的基础上,过表达SNF1可以进一步提高面包酵母的麦芽糖代谢和不加糖面团发酵水平,同时不影响面包酵母菌株生长性能,因此转化子BYKPS-R具备潜在的工业应用价值,同时该研究为快速发酵面包酵母菌株的选育奠定基础。
[Abstract]:The effects of protein phosphatase (PP1) on maltose metabolism and non-sugar dough fermentation of baker's yeast by REG1 deletion and overexpression of protein kinase gene SNF1 were studied. A comparative analysis of REG1 deletion and overexpression of SNF1 was carried out. The level of MAL gene m RNA in the mutant BYK-R of parent strain BY14 伪 BYKPS-R and parent strain BY14 伪 + REG1 was detected. Maltase and maltose permeation enzyme activity, maltose metabolism level, fermentation ability of dough without sugar, and basic growth performance. The results showed that knockout of REG1 and overexpression of SNF1 could significantly improve the transcription and enzyme activity of genes related to maltose metabolism. The maltose consumption rate of bread yeast was increased by 18.59% and 4.40% than that of BY14 伪 and BYK-R, respectively, and the fermentability of dough without sugar was increased by 12.51% and 3.22%, respectively. On the basis of REG1 gene deletion, the maltose consumption rate of bread yeast was increased by 18.59% and 4.40% than that of BY14 伪 and BYK-R, respectively. Overexpression of SNF1 can further improve the maltose metabolism of bread yeast and the fermentation level of dough without adding sugar without affecting the growth performance of bread yeast strain, so the transformant BYKPS-R has potential industrial application value. At the same time, this study laid a foundation for the rapid fermentation of bread yeast strain selection.
【作者单位】：工业微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院;
【基金】：国家自然科学基金项目(31571809,31171730) 天津科技大学2015年优秀博士学位论文创新基金资助(201503)
【分类号】：TS213.21

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