圈养虎源大肠杆菌QRDR基因检测及分析

发布时间：2018-03-22 23:36

  本文选题：虎源大肠杆菌　切入点：喹诺酮耐药决定区(QRDR)　出处：《黑龙江畜牧兽医》2017年16期 　论文类型：期刊论文

【摘要】：为了解大肠杆菌对氟喹诺酮类药物的耐药机制和喹诺酮耐药决定区GyrA、GyrB、ParC、ParE四种基因的流行情况,采用聚合酶链式反应(PCR)技术对30株虎源大肠杆菌的耐药菌株进行了氟喹诺酮类药物耐药基因的检测,并对目的片段进行测序分析。结果表明:GyrA、GyrB、ParC、ParE阳性率分别为40.00%、63.33%、63.33%、40.00%;GyrA亚基发生Ser83→Leu、Asp87→Asn、Glu214→Gly的突变,GyrB亚基发生Ser195→Asn的突变,ParC亚基上氨基酸未发生取代,ParE亚基发生Ser85→Ala的突变。说明GyrA、GyrB亚基上发生的氨基酸替代是耐药菌对氟喹诺酮类药物产生耐药性的主要机制之一。
[Abstract]:In order to understand the resistance mechanism of Escherichia coli to fluoroquinolones and the prevalence of four genes in GyrAgyr GyrBfus Parc Pare, a quinolone resistance determinant, Polymerase chain reaction (PCR) technique was used to detect fluoroquinolone-resistant genes in 30 strains of Escherichia coli from tiger origin, and the target fragment was sequenced. The results showed that the positive rate of Ser83 was 40.0063.3340.0063.3343. 鈫扡eu,Asp87. 鈫扐sn,Glu214. 鈫扥ccurrence of Ser195 by mutation of GyrB subunit in Gly. 鈫扵he amino acids on the mutant Parc subunit of Asn have not replaced the Pare subunit to produce Ser85. 鈫扵he mutation of Ala suggests that amino acid substitution on GyrB subunit is one of the main mechanisms of resistance of resistant bacteria to fluoroquinolones.
【作者单位】： 东北林业大学野生动物资源学院;
【基金】：东北林业大学大学生创新训练计划项目(201610225219)
【分类号】：S852.612
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本文编号：1650929