绵羊MUSTN1基因的克

发布时间：2018-03-23 03:16

本文选题：MUSTN　切入点：绵羊　出处：《浙江农业学报》2017年10期 　论文类型：期刊论文

【摘要】：为了探讨绵羊(Ovis aires)MUSTN1基因特征及其在不同发育阶段的背最长肌与股二头肌组织中的表达规律,选取0、2、6、12和24月龄健康绵羊15只,每组3只,采取背最长肌和股二头肌组织,应用分子生物学等方法,对MUSTN1基因CDS区进行克隆,对其进行生物信息学和组织表达分析。结果表明,MUSTN1基因CDS区全序列为248 bp,编码82个氨基酸,其分子量为8.89 ku,等电点为9.93,GC含量大于AT含量,含有5个磷酸化位点、5个N-糖基化位点、1个O-糖基化位点、0个跨膜结构、疏水性蛋白,二级结构主要以无规卷曲为主;与山羊(Capra hircus)该基因核苷酸以及编码氨基酸序列的同源性最高,分别为99.2%、100%,其次为牛(Bos taurus),分别为97.2%、100%。MUSTN1基因在绵羊不同发育阶段(0、2、6、12和24月龄)的背最长肌与股二头肌中均有表达,在背最长肌中,24月龄MUSTN1表达量最高,极显著高于其他月龄(P0.01),0月龄MUSTN1表达量最低,极显著低于2、12月龄(P0.01),显著低于6月龄(P0.05);而在6月龄绵羊股二头肌中MUSTN1表达量最高且极显著高于其他月龄(P0.01),24月龄表达量次之且极显著高于12月龄(P0.01),显著高于0月龄(P0.05)。结论为绵羊MUSTN1基因的编码区序列在物种间具有保守性,且在不同发育阶段的背最长肌和股二头肌组织中均表达,其表达量的高低可能与骨骼肌肉的生长快慢相关。
[Abstract]:In order to investigate the characteristics of Ovis aires)MUSTN1 gene and its expression in the longissimus dorsi and biceps femoris at different developmental stages, 15 healthy sheep of 12 and 24 months of age were selected, 3 in each group, and 3 in each group. The longissimus dorsi muscle and biceps femoris muscle were used. The CDS region of MUSTN1 gene was cloned by molecular biology, and its bioinformatics and tissue expression analysis were carried out. The results showed that the whole sequence of CDS region of MUSTN1 gene was 248bp, encoding 82 amino acids. Its molecular weight is 8.89 ku. the content of isoelectric point is 9.93% GC is higher than that of AT, and it contains 5 phosphorylation sites, 5 N-glycosylation sites, 1 O-glycosylation site, 0 transmembrane structure, hydrophobic protein and secondary structure. The nucleotide sequence and amino acid sequence of Capra hircus gene had the highest homology, 99.2 and 100, respectively, followed by Bos taurusus, which was 97.2and 100.MUSTN1 gene was expressed in the longissimus dorsi muscle and biceps femoris muscle at different developmental stages of 12 and 24 months of age, respectively. In the longissimus dorsi muscle, the highest expression of MUSTN1 was observed at 24 months old, which was significantly higher than that in the other months of age. The expression of MUSTN1 in the biceps femoris femoris was the highest and significantly higher in the biceps femoris than that in the other months of age, and was significantly higher than that of the 12-month-old P0.01, and was significantly higher than that of the 12-month-old P0.01M, and was significantly higher than that of the 0-month-old sheep, and was significantly lower than that of the 6-month-old sheep's biceps femoris (P0.01), which was significantly higher than that of the 6-month-old sheep's biceps femoris. Conclusion the coding region of sheep MUSTN1 gene is conserved among species. The expression level of longissimus dorsi muscle and biceps femoris muscle may be related to the growth rate of skeletal muscle.
【作者单位】：甘肃农业大学动物科学技术学院;甘肃肉羊繁育生物技术工程实验室;甘肃农业大学动物医学院;
【基金】：甘肃省科技重大专项计划(1602NKDH020) 甘肃农业大学校级课题(GSAU-ZL-2015-030) 国家肉羊产业技术体系项目(CARS-38)
【分类号】：S826

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