利用全基因组重测序分析鹿茸重量相关基因

发布时间：2018-03-23 17:35

  本文选题：全基因组重测序　切入点：梅花鹿　出处：《遗传》2017年11期

【摘要】：茸角是鹿科动物特有的器官,具有重要的生物学意义。鹿茸生长是一个复杂的生物代谢过程,其重量与遗传因素有一定关联。本研究对饲养条件基本一致的5个梅花鹿(Cervus nippon)群体进行调查,获得高产和低产梅花鹿个体共100只,利用全基因组重测序分析这些个体与鹿茸重量相关的遗传变异。结果表明,共得到94个与鹿茸重量可能相关的遗传变异,其中有2个变异位点分别定位于OAS2和ALYREF/THOC4基因的外显子区,且ALYREF/THOC4基因在鹿茸中表达量很高。功能富集分析发现,这些遗传变异与鹿茸生长发育密切相关,可作为潜在的鹿茸重量相关遗传变异。本研究首次通过全基因组重测序直接筛选与鹿茸重量相关的遗传变异,并分析关联基因的生物学功能,对揭示鹿茸生长发育和鹿茸重量差异形成的遗传机制具有重要意义。
[Abstract]:Antlers is a unique Cervidae animal organ, has important biological significance. Antler growth is a complex process of biological metabolism, is related to its weight and genetic factors. In this study, 5 deer breeding conditions consistent (Cervus nippon) groups are investigated to obtain high yield and low yield of sika deer a total of 100. Analysis of these individuals associated with antler weight genetic variation by whole genome sequencing. The results show that there are 94 possible and antler weight related genetic variants, of which 2 mutation sites were located on OAS2 and ALYREF/THOC4 gene, and ALYREF/THOC4 gene in antler expression is very high. The function of enrichment the analysis found that the genetic variation and antler growth and development are closely related, can be used as a potential genetic variation of antler weight for the first time. In this study, whole genome sequencing directly through the heavy screen Selecting the genetic variation related to pilose antler weight and analyzing the biological function of related genes are important for revealing the genetic mechanism of pilose antler growth and antler weight difference.

【作者单位】： 中国农业科学院特产研究所特种经济动物分子生物学国家重点实验室农业部特种经济动物遗传育种与繁殖重点实验室;中国农业科学院深圳农业基因组研究所;吉林农业大学;
【基金】：吉林省科技发展计划项目(编号:20150309006YY)资助~~
【分类号】：S825
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本文编号：1654481