沉默MCM7基因后肝癌SMMC-7721细胞差异表达基因的筛选

发布时间：2018-03-23 18:12

本文选题：肝癌　切入点：MCM　出处：《世界华人消化杂志》2016年10期

【摘要】：目的:探究微小染色体维持蛋白7(minichromosome maintenance protein 7,MCM7)基因在调节肝癌细胞生长的相关机制.方法:通过s i R N A干扰技术沉默人肝癌SMMC-7721细胞中的MCM7基因表达,采用人全基因组表达谱芯片,筛查MCM7沉默后差异表达基因,进行生物信息学分析,并对部分差异表达基因进行蛋白免疫印迹法(Western blot)验证.结果:芯片筛选出在人肝癌SMMC-7721细胞中沉默MCM7基因后差异表达基因1010个,其中上调基因391个,下调基因619个.这些基因主要涉及生物大分子代谢、细胞周期调控、细胞增殖、细胞凋亡等方面,参与胞吞、肿瘤相关通路、P53信号通路、mTOR信号通路等通路的改变.Western blot对部分差异基因表达验证的结果显示CCND1、SKP2和JUP蛋白表达均下调,与芯片检测结果一致.结论:应用人基因表达谱芯片,成功筛选出沉默MCM7基因后人肝癌细胞SMMC-7721差异表达基因,为探究MCM7基因影响肝癌细胞生长提供了有效线索.
[Abstract]:Aim: to investigate the mechanism of the microchromosome maintenance protein 7(minichromosome maintenance protein 7 (MCM7) gene in regulating the growth of hepatocellular carcinoma cells. Methods: the expression of MCM7 gene in human hepatocellular carcinoma SMMC-7721 cells was silenced by sirna interference technique. Human genome expression microarray was used to screen differentially expressed genes after MCM7 silencing and bioinformatics analysis was carried out. Results: 1010 differentially expressed genes were screened by microarray after silencing MCM7 gene in human hepatocellular carcinoma SMMC-7721 cells, including 391 up-regulated genes. These genes are mainly involved in the metabolism of biomolecules, cell cycle regulation, cell proliferation, apoptosis and so on. Tumor related Pathway: p53 signaling Pathway / mTOR signaling Pathway. The results of partial differential gene expression verification by Western blot showed that the expression of CCND1- SKP2 and JUP were down-regulated, which was consistent with the results of microarray detection. Conclusion: human gene expression microarray was used. The differentially expressed SMMC-7721 genes of human hepatoma cells with silencing MCM7 gene were successfully screened, which provided an effective clue to explore the effect of MCM7 gene on the growth of HCC cells.
【作者单位】：广西医科大学附属肿瘤医院实验研究部;广西医科大学研究生学院;
【基金】：国家自然科学基金资助项目,No.81260080 广西自然科学基金资助项目,No.2013GXNSFAA019210~~
【分类号】：R735.7

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