中国大陆1993～2013年流行的麻疹病毒H1基因型血凝素基因变异特征

发布时间：2018-03-24 13:17

本文选题：麻疹病毒　切入点：血凝素　出处：《病毒学报》2017年02期

【摘要】：为探讨2013年引起中国大陆麻疹复发的麻疹野病毒中和抗原血凝素(Hemagglutinin,HA)的变异动态,本研究对1993~2013年中国大陆流行的麻疹病毒H1基因型血凝素(HA)编码基因和氨基酸变异进行系统分析,为评价中国疫苗对2013年流行的麻疹野病毒的保护效果提供科学依据。选取2013年8株麻疹病毒代表株,扩增HA基因编码区序列(1 854bp)并进行序列测定和分析。从GenBank上下载45株1993~2012年中国流行株、中国疫苗株上海191(S-191)和长春47(C-47)和24株WHO参考株HA基因编码区序列,MEGA5.05构建亲缘关系树和进行氨基酸位点比对,分析重要中和抗原位点的变异。使用BioEdit进行同源性关系分析。根据HA基因序列的亲缘性关系分析,2013年麻疹病毒代表株均位于H1a亚型的第3分支,且2000年以后分离的H1a亚型的麻疹野毒株均属于该分支;8株2013年麻疹病毒代表株核苷酸和氨基酸的同源性分别为97.6%~99.8%和97.8%~100.0%,与1993~2012年H1a基因亚型麻疹病毒的核苷酸和氨基酸同源性分别为97.6%~99.6%和97.0%~99.6%,与中国疫苗株S-191和C-47的核苷酸和氨基酸同源性分别为94.3%~94.7%和94.6%~95.4%;与中国疫苗株S-191原型野病毒氨基酸序列比对,1993~2013年麻疹病毒代表株HA主要中和抗原位点未发生重大变异,但包括2013年代表株的近年流行的H1a麻疹毒株在氨基酸第240位由丝氨酸突变成了天冬酰胺(Ser240Asn),导致潜在的糖基化位点NLS238~240的缺失,但H1基因型麻疹病毒均保留有4个潜在的糖基化位点。中国大陆流行的麻疹病毒H1基因型在重要的中和抗原位点均保持稳定,中国使用的麻疹疫苗对中国现流行的麻疹病毒H1a亚型具有较好的保护效果。中国应继续加强流行麻疹病毒血凝素基因的动态监测,以评价我国疫苗对流行麻疹野病毒的保护效果,为我国消除麻疹提供疫苗使用的科学依据。
[Abstract]:In order to investigate the variation dynamics of hemagglutinin (HA), a wild measles virus neutralizing antigen, which caused measles recurrence in mainland China in 2013, the coding gene and amino acid variation of measles virus H1 genotype hemagglutinin (H1) in mainland China from 1993 to 2013 were systematically analyzed. In order to provide scientific basis for evaluating the protective effect of Chinese vaccine on wild measles virus in 2013, 8 representative measles virus strains were selected in 2013. The coding region of HA gene was amplified and sequenced and analyzed. 45 epidemic strains from 1993 to 2012 were downloaded from GenBank. Chinese vaccine strains Shanghai 191 S-191) and Changchun 47 C 47) and 24 WHO reference strains of HA gene coding region sequence of MEGA 5.05 constructed a phylogenetic tree and amino acid site alignment. BioEdit was used for homology analysis. According to the genetic relationship analysis of HA gene sequence, the representative strains of measles virus in 2013 were all located in the third branch of H1 a subtype. And the nucleotide and amino acid homology of all the wild measles viruses isolated after 2000 were 97.699.8% and 97.8%, respectively, which were similar to the nucleotides and amino acids of H1 a gene subtype measles virus from 1993 to 2012. The homology of nucleotide and amino acid of the representative strains of measles virus in 2013 were 97.699.8% and 97.8100.0, respectively, which were similar to those of H1 a gene subtype measles virus from 1993 to 2012. The nucleotide and amino acid homology with Chinese vaccine strain S-191 and C-47 were 94.33.7% and 94.6%, respectively. The amino acid sequence of Chinese vaccine strain S-191 prototype wild virus was compared with the amino acid sequence of Chinese vaccine strain S-191. There was no significant variation in the site. However, the recently prevalent H1N1 measles strain, including the representative strain of 2013, changed from serine process to Ser240Asnn in amino acid at 240th, resulting in the deletion of potential glycosylation site NLS238~240. However, the H1 genotype of measles virus had four potential glycosylation sites. The H1 genotype of measles virus in mainland China remained stable at the important neutralizing antigen sites. The measles vaccine used in China has a good protective effect on the current epidemic measles virus H1N1 subtype in China. China should continue to strengthen the dynamic surveillance of the hemagglutinin gene of the epidemic measles virus. In order to evaluate the protective effect of Chinese vaccine on epidemic measles wild virus and provide scientific basis for measles elimination in China.
【作者单位】：中国疾病预防控制中心病毒病预防控制所卫生部医学病毒病重点实验室世界卫生组织西太平洋区麻疹参比实验室;
【基金】：国家自然科学基金(项目号:81371791),题目:麻疹病毒血凝素蛋白分子进化、抗原变异及其对我国疫苗保护效果的影响
【分类号】：R511.1

【相似文献】