玉米ZmCPK9基因在非生物胁迫下的表达分析

发布时间：2018-03-25 02:15

本文选题：玉米　切入点：CPK基因　出处：《新疆农业科学》2017年09期

【摘要】：【目的】钙依赖蛋白激酶(CPKs)是一类依赖于Ca~(2+)的丝氨酸/苏氨酸蛋白激酶,仅在植物和部分低等生物中存在。Ca~(2+)作为第二信使,在植物生长发育和抗逆应答过程中起着重要作用。研究玉米Zm CPK9基因在幼苗期不同逆境胁迫下的动态表达,为分析该基因在玉米逆境应答中的功能和机制奠定基础。【方法】利用生物信息学方法,分析Zm CPK9基因序列特征,采用Real-time PCR技术分析Zm CPK9在干旱、低温、盐和ABA诱导下的表达特征。【结果】利用生物信息学方法从玉米基因组中鉴定出CPK基因,命名为ZmCPK9。序列分析表明Zm CPK9 c DNA序列长1 548 bp,编码515个氨基酸。生物信息学分析显示,在蛋白质序列和结构上该基因与其他物种CPK基因一样非常保守。Real-time PCR的结果表明,在干旱、低温、盐和ABA诱导下,Zm CPK9表达量均有所上调。【结论】Zm CPK9是玉米CPKs基因家族中一个新的成员,对干旱、低温、盐和ABA等非生物胁迫具有应答反应,推测Zm CPK9对植物防御非生物胁迫具有一定作用。
[Abstract]:[objective] Ca-dependent protein kinase (Ca~(2) is a serine / threonine protein kinase, which exists only in plants and some lower organisms as the second messenger. Zm CPK9 gene expression in maize under different stress stress in seedling stage was studied. In order to analyze the function and mechanism of Zm CPK9 gene in maize stress response. [methods] Zm CPK9 gene sequence characteristics were analyzed by bioinformatics, and Real-time PCR technique was used to analyze Zm CPK9 in drought and low temperature. Expression characteristics induced by salt and ABA. [results] CPK gene was identified from maize genome by bioinformatics method, named ZmCPK9. sequence analysis showed that Zm CPK9 c DNA sequence was 1 548 BP, encoding 515 amino acids. The protein sequence and structure of the gene are as conserved as the CPK gene of other species. Real-time PCR results show that in drought, low temperature, [conclusion] Zm CPK9 is a new member of CPKs gene family in maize, which is responsive to abiotic stresses such as drought, low temperature, salt and ABA. Zm CPK9 may play an important role in plant defense against abiotic stress.
【作者单位】：新疆农业科学院核技术生物技术研究所;
【基金】：国家自然科学基金项目(31260331) 新疆农业科学院优秀青年科技人才基金项目(2014026)~~
【分类号】：S513

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