胡萝卜抗坏血酸过氧化物酶基因的分离及其对非生物胁迫的响应

发布时间：2018-03-25 22:26

本文选题：抗坏血酸过氧化物酶　切入点：基因克隆　出处：《南京农业大学学报》2016年01期

【摘要】：[目的]抗坏血酸过氧化物酶(APX)利用抗坏血酸作为电子供体,清除植物中的过氧化物,在植物应对非生物胁迫中起重要作用。本文目的是研究胡萝卜Dc APX基因在抵御逆境胁迫过程中的响应情况。[方法]以胡萝卜‘黑田五寸’为研究材料,克隆获得胡萝卜Dc APX基因(Gen Bank登录号为KR364573),对该基因进行序列分析,并研究其主要非生物胁迫(高温、低温、干旱和盐胁迫)下的基因表达情况。[结果]序列分析表明,胡萝卜Dc APX基因全长753个碱基,编码250个氨基酸,预估其蛋白质相对分子质量为27.76×103,p I值5.65。进化分析表明,胡萝卜Dc APX在进化关系上与同属伞形科的短果茴芹同源性最高。空间结构分析表明,胡萝卜Dc APX的氨基酸二级结构由38.40%的α-螺旋、10.40%的延伸主链和51.20%的随机卷曲构成。胡萝卜Dc APX的氨基酸三级结构由10个α-螺旋和4个β-折叠构成。实时荧光定量PCR结果显示,胡萝卜‘黑田五寸’中Dc APX基因表达具有组织特异性,在叶中表达量最高。[结论]胡萝卜中Dc APX基因可以响应多种非生物逆境胁迫,在胡萝卜非生物逆境胁迫调控过程中起着重要作用。
[Abstract]:[objective] ascorbic acid peroxidase (APX) used ascorbic acid as an electron donor to remove peroxides from plants. The purpose of this paper is to study the response of carrot DC APX gene to stress stress. [methods] Carrot 'black field five inches' was used as the research material. The accession number of Genin Bank of carrot DC APX gene was KR364573. The gene was sequenced, and the gene expression was studied under abiotic stress (high temperature, low temperature, drought and salt stress). [results] sequence analysis showed that, Carrot DC APX gene is 753 bases in length and encodes 250 amino acids. The relative molecular weight of the protein is estimated to be 27.76 脳 10 ~ 3p _ I 5.65. The phylogenetic relationship of DC APX in carrot is the highest with anise, which belongs to the same umbrella family, and the spatial structure analysis shows that, The amino acid secondary structure of carrot DC APX consists of 38.40% 伪 -helix 10.40% extended main chain and 51.20% random crimp. The amino acid tertiary structure of carrot DC APX is composed of 10 伪 -helix and 4 尾 -folds. The results of real-time fluorescence quantitative PCR show that the amino acid secondary structure of DC APX is composed of 10 伪 -helix and 4 尾 -fold. The expression of DC APX gene in carrot 'black field five inches' has tissue specificity and the highest expression level in leaves. [conclusion] DC APX gene in carrot can respond to various abiotic stresses. It plays an important role in the regulation of abiotic stress in carrot.
【作者单位】：南京农业大学园艺学院/作物遗传与种质创新国家重点实验室/农业部华东地区园艺作物生物学与种质创制重点实验室;
【基金】：教育部新世纪优秀人才支持计划项目(NCET-11-0670) 江苏省杰出青年基金项目(BK20130027) 江苏高校优势学科建设项目(2011PAPD)
【分类号】：S631.2

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