福建黄兔肌纤维性状及Myh6基因表达水平分析

发布时间：2018-03-26 00:15

本文选题：福建黄兔　切入点：肌纤维　出处：《浙江大学学报(农业与生命科学版)》2017年03期

【摘要】：选取我国地方品种福建黄兔为研究对象,利用冰冻切片技术对不同发育阶段福建黄兔背肌和腿肌的肌纤维性状进行研究,通过荧光定量聚合酶链式反应技术分析在肌肉发育过程中起重要作用的Myh6基因在福建黄兔不同发育阶段表达水平的差异,并与肌纤维性状进行相关性分析。结果显示:2、4、6、8、10和12周龄福建黄兔的白肌纤维密度均显著大于红肌纤维和中间型肌纤维,白肌纤维的面积显著高于红肌纤维,长径和短径的大小排列为白肌纤维中间型肌纤维红肌纤维,但差异无统计学意义;在6周龄前,背肌和腿肌中各类肌纤维长径、短径和面积呈显著增大的趋势,而6周龄后增大趋势逐渐变缓;Myh6基因在公母兔背部和腿部的表达均呈先上升后下降的趋势,其中母兔在4周龄表达量最高,公兔在6周龄表达量最高;Myh6基因的表达量与肌纤维面积、长径和短径的变化一致。表明Myh6基因与肌纤维类型和肌纤维的生长发育密切相关。
[Abstract]:The muscle fiber properties of the dorsal and leg muscles of Fujian yellow rabbits at different developmental stages were studied by using frozen section technique. The expression levels of Myh6 gene, which plays an important role in muscle development, were analyzed by fluorescence quantitative polymerase chain reaction (FQPCR) in Fujian yellow rabbits at different developmental stages. The results showed that the density of white muscle fiber in 10 and 12 week old Fujian yellow rabbits was significantly larger than that of red muscle fiber and intermediate muscle fiber, and the area of white muscle fiber was significantly higher than that of red muscle fiber. The size of length and short diameter were arranged as white muscle fiber, middle type muscle fiber red muscle fiber, but the difference was not statistically significant. Before 6 weeks of age, the length, short diameter and area of all kinds of muscle fibers in dorsal muscle and leg muscle were significantly increased. The expression of Myh6 gene in the back and legs of male and female rabbits increased first and then decreased after 6 weeks of age, and the expression of Myh6 gene was the highest in female rabbits at 4 weeks of age. The expression of Myh6 gene in male rabbits at 6 weeks of age was consistent with the changes of muscle fiber area, length and short diameter, indicating that Myh6 gene was closely related to the type of muscle fiber and the growth and development of muscle fiber.
【作者单位】：扬州大学动物科学与技术学院江苏省动物繁育与分子设计重点实验室;
【基金】：国家现代农业产业技术体系建设专项资金(CARS-44-1) 江苏省高校优势学科建设工程项目(PAPD 2014-134)
【分类号】：S829.1

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