电针“夹脊”穴预处理对心肌缺血再灌注大鼠细胞色素P450信号通路基因表达的影响

发布时间：2018-03-26 10:17

本文选题：电针　切入点：预处理　出处：《针刺研究》2017年01期

【摘要】：目的:研究电针"夹脊"穴预处理对心肌缺血再灌注损伤大鼠的心肌保护作用及对心肌细胞色素P 450(CYP 450)信号通路基因表达的影响。方法:Wistar大鼠随机分为假手术组、模型组、夹脊组、内关组、阳陵泉组、曲池组,每组10只。采用结扎左冠状动脉前降支法复制心肌缺血再灌注损伤模型。各电针预处理组造模前针刺相应穴位30min,每天1次,治疗7d。观察各组造模前后ST段位移值,ELISA法检测血清肌酸激酶同工酶(CK-MB)的活性,微板法检测血清乳酸脱氢酶(LDH)活性,RT-PCR法检测心肌CYP 450相关基因cyp 1a1、cyp 2b2、cyp 2c11、cyp 4a2mRNA表达量的变化。结果:心电图ST段位移值变化,结扎30min后,夹脊组、内关组均显著低于模型组(P0.01);再灌注30min后,内关组与模型组比较显著降低(P0.01)。与假手术组比较,模型组血清CK-MB及LDH活性均显著升高(P0.01);与模型组比较,夹脊组、内关组、阳陵泉组血清CK-MB及LDH活性均显著降低(P0.01);与夹脊组比较,曲池组血清LDH显著升高,曲池组及阳陵泉组血清CK-MB显著升高(P0.05)。模型组与假手术组比较,心肌cyp 1a 1、cyp 2b2mRNA表达量明显升高(P0.01),cyp 2c11mRNA表达量降低(P0.01);夹脊组、内关组、阳陵泉组与模型组比较,cyp 1a1、cyp 4a2mRNA表达量降低(P0.01),cyp 2b2、cyp 2c11mRNA表达量升高(P0.01);内关组与夹脊组比较,cyp 1a1mRNA表达量降低,cyp 2b2mRNA表达量升高(P0.05)。结论:电针"夹脊"穴预处理能够减少心肌缺血再灌注中血清心肌酶的产生,降低心电图ST段,上调心肌CYP 450表氧化酶cyp 2b2、cyp 2c11mRNA表达量,下调ω-羟化酶cyp 1a1、cyp 4a2mRNA表达量,从而起到心肌保护作用。
[Abstract]:Objective: to study the protective effect of electroacupuncture (EA) at Jiaji point on myocardial protection and the expression of cytochrome P 450(CYP 450) signal pathway gene in myocardial ischemia-reperfusion injury rats. Methods: the rats were randomly divided into sham operation group and model group. Jiaji group, Neiguan group, Yanglingquan group, Quchi group, 10 rats in each group. Myocardial ischemia-reperfusion injury model was established by ligating the anterior descending branch of left coronary artery. The corresponding acupoints were needled once a day for 30 mins in each electroacupuncture preconditioning group. After 7 days of treatment, the activity of serum creatine kinase isoenzyme (CK-MBB) was detected by Elisa before and after the establishment of the model. Detection of serum lactate dehydrogenase (LDH) activity by microplate method and RT-PCR method were used to detect the expression of CYP 450 related gene cyp 1a 1a 1, cyp 2b 2, cyp 2c 11 cyp 4a2mRNA. Results: St segment displacement of electrocardiogram (ECG) changed, and after ligation of 30min, the expression of St segment displacement was detected. Compared with the sham operation group, the serum CK-MB and LDH activities of the model group were significantly higher than those of the model group, and compared with the model group, the activities of serum CK-MB and LDH in the model group were significantly higher than those in the model group, and compared with that in the model group, the activities of serum CK-MB and LDH in the Neiguan group were significantly lower than those in the model group. The activities of serum CK-MB and LDH in Yanglingquan group were significantly lower than those in Jiaji group, and the serum LDH level in Quchi group was significantly higher than that in jiaji group, while the serum CK-MB level in Quchi group and Yanglingquan group was significantly higher than that in sham operation group. The expression of cyp 1a 1 2b2mRNA in myocardium was significantly increased and the expression of cyp 2c11mRNA was decreased in P0.01a group, Neiguan group, Jiaji group, and Neiguan group. Compared with the model group, the expression of cyp 1a 1 4a2mRNA in Yanglingquan group was lower than that in the model group, and the expression of cyp 2b 2 2c11mRNA was increased in Neiguan group and Jiaji group compared with the model group. Conclusion: electroacupuncture at Jiaji point preconditioning can reduce the expression of cyp 2b2mRNA in myocardium of Neiguan group and Jiaji group. Conclusion: electroacupuncture at Jiaji point preconditioning can reduce the expression of cyp 2b2mRNA in myocardium. The production of serum myocardial enzymes during ischemia-reperfusion, The St segment of ECG was decreased, the expression of CYP 450 oxidase cyp 2b2 2c11mRNA was up-regulated, and the expression of 蠅 -hydroxylase cyp 1a 1 cyp 4a2mRNA was down-regulated so as to play a protective role in myocardium.
【作者单位】：山东中医药大学针灸推拿学院;山东中医药大学附属医院针灸科;
【基金】：国家自然科学基金项目(No.81373722) 山东省自然基金联合专项(No.ZR 2014HL 100)
【分类号】：R245.97

【参考文献】