Nme2基因参与小鼠基质细胞蜕膜化过程

发布时间：2018-03-26 10:37

本文选题：子宫内膜　切入点：蜕膜化　出处：《重庆医科大学学报》2017年08期

【摘要】：目的:检测Nme2(nucleoside diphosphate kinase 2)基因在小鼠早期妊娠和人工诱导蜕膜化的子宫内膜组织中的表达规律;探究Nme2基因与子宫基质细胞蜕膜化的相关性。方法:利用免疫组织化学、Western blot和实时荧光定量PCR(q RTPCR)分别检测小鼠妊娠第5天着床点(D5IS)、着床旁(D5IIS),D6IS、D6IIS、D8IS、D8IIS及人工诱导蜕膜化模型小鼠子宫内膜中Nme2的表达定位,m RNA和蛋白质水平的表达水平。结果:小鼠D5、D6、D8子宫内膜中着床点Nme2基因的m RNA和蛋白水平的表达明显高于着床旁;免疫组织化学结果显示Nme2表达定位表达于D5着床点的初级蜕膜区(primary decidua region,PDZ)、D6和D8着床点的次级蜕膜区(secondary decidua region,SDZ);人工诱导蜕膜化模型中,Nme2基因的m RNA和蛋白质水平在诱导组的表达明显高于对照组。结论:Nme2基因的表达上调与小鼠基质细胞的蜕膜化过程相关。
[Abstract]:Objective: to investigate the expression of Nme2(nucleoside diphosphate kinase 2 (Nme2(nucleoside diphosphate kinase 2) gene in early pregnancy and decidualized endometrium of mice. To explore the relationship between Nme2 gene and decidualization of uterine stromal cells. Methods: immunohistochemical blot and real time fluorescence quantitative PCR(q were used to detect D5ISN, D5IISN, D6ISD6IIS-D8ISD8IIS and artificial induced decidualization model in mice on the 5th day of pregnancy, respectively. Results: the expression of m RNA and protein of Nme2 gene at implantation site was significantly higher in mouse endometrium than that near implantation site. Immunohistochemical results showed that the expression of Nme2 was localized in primary decidua decidua region of D5 implantation site and secondary decidua region of D8 implantation site, and m RNA and protein levels of Nme2 gene were induced in artificial decidualization model. Conclusion the upregulation of the expression of the 1: Nme2 gene is related to the process of decidualization of mouse stromal cells.
【作者单位】：重庆医科大学公共卫生与管理学院生殖生物学研究室;重庆医科大学中医药学院中药综合教研室;
【基金】：国家自然科学基金资助项目(编号:81671493)
【分类号】：R714

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