碳源对StSte12基因调控玉米大斑病菌生长和分生孢子发育的影响

发布时间：2018-03-28 13:59

本文选题：玉米大斑病菌　切入点：StSte基因　出处：《河南农业科学》2016年01期

【摘要】：以葡萄糖、蔗糖、乳糖和可溶性淀粉为碳源分别培养玉米大斑病菌野生型和St Ste12基因RNAi阳性转化子,研究碳源对St Ste12基因调控玉米大斑病菌菌丝生长和分生孢子发育的影响,为阐明St Ste12基因的功能和病菌的致病机制奠定基础。结果表明,St Ste12基因对玉米大斑病菌菌丝生长和分生孢子发育均有重要的调控作用,St Ste12基因的表达量下降后,菌丝的生长势变弱,颜色变浅,生长速度显著下降,产孢量显著降低。4种供试碳源中,可溶性淀粉对St Ste12基因调控菌丝生长和发育的影响最大,蔗糖最小;乳糖对St Ste12基因调控分生孢子发育没有显著影响,而其他3种碳源的影响均达到显著水平。
[Abstract]:Glucose, sucrose, lactose and soluble starch were used as carbon sources to culture wild type and St Ste12 gene RNAi positive transformants of maize spotted spot fungus. The effects of carbon source on the regulation of mycelium growth and conidial development by St Ste12 gene were studied. In order to clarify the function of St Ste12 gene and the pathogenic mechanism of the pathogen, the results showed that St Ste12 gene had an important role in regulating mycelium growth and conidial development of Corn spotted spot, and the expression of St Ste12 gene was decreased after the decrease of the expression of St Ste12 gene. The growth potential of mycelium became weaker, the color became lighter, the growth rate decreased significantly, and the spore production decreased significantly. Among the 4 carbon sources tested, soluble starch had the greatest effect on the regulation of hyphal growth and development by St Ste12 gene, and sucrose was the smallest. Lactose had no significant effect on the conidial development of St Ste12 gene, but the effect of the other three carbon sources was significant.
【作者单位】：唐山师范学院生命科学系;
【基金】：国家自然科学基金项目(31271997) 河北省自然科学基金项目(C2014105067) 唐山师范学院团队建设基金项目(2014E04)
【分类号】：S435.131

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