鹌鹑野灰相关蛋白基因外显子Ⅰ的多态性分析

发布时间：2018-03-28 17:04

本文选题：鹌鹑　切入点：野灰相关蛋白基因　出处：《中国畜牧兽医》2017年05期

【摘要】：家禽的羽色遗传一直是炙手可热的研究课题,因为羽色是禽类一种重要的质量性状。本研究利用聚丙烯凝胶电泳技术对不同羽色的4个鹌鹑群体(中国黄羽鹌鹑、黑羽鹌鹑、朝鲜鹌鹑、北京白羽鹌鹑)进行野灰相关蛋白基因(agouti-related protein,Agrp)外显子Ⅰ的多态性检测,探讨Agrp外显子Ⅰ与鹌鹑羽毛颜色的关系,为鹌鹑的育种及生产提供参考。结果表明,Agrp外显子Ⅰ在中国黄羽鹌鹑、黑羽鹌鹑、朝鲜鹌鹑、北京白羽鹌鹑群体中都检测到了2个等位基因,分别用A和B表示。等位基因A在北京白羽鹌鹑中的基因频率最高,达到了0.9500。B等位基因在朝鲜鹌鹑中的频率最高,达到了0.3392,在北京白羽鹌鹑中的基因频率最低,只有0.0500。基因型AA在北京白羽鹌鹑中的基因型频率最高(0.9500),在朝鲜鹌鹑和黑羽鹌鹑中最低(0.4286)。基因型AB在北京白羽鹌鹑中没有出现。测序发现,在黑羽鹌鹑和朝鲜鹌鹑中有AG的杂合位点,而在北京白羽鹌鹑中只有G位点。推测Agrp外显子Ⅰ的AB基因型(或B等位基因)与鹌鹑的黑羽色可能有一定关系。
[Abstract]:The inheritance of feather color in poultry has been a hot research topic, because the feather color is an important quality trait of poultry. In this study, four quail populations (Chinese yellow quail and black quail) with different feather colors were studied by using polypropylene gel electrophoresis (PP gel electrophoresis). Korean quails and Beijing white quail) were detected for the polymorphism of exon I of the wild gray associated protein gene (agouti-related protein), and to explore the relationship between Agrp exon I and quail feather color. The results showed that two alleles were detected in Chinese yellow quail, black quail, Korean quail and Beijing white quail population. The frequency of allele A was the highest in Beijing white quail, the highest allele was 0.9500.B in Korean quail, and the frequency of allele A was 0.3392, and the frequency of allele A was the lowest in Beijing white quail. The genotype AA was the highest in Beijing white quail, and the lowest in Korean quail and black quail. Genotype AB was not found in Beijing white quail. AG heterozygous loci were found in black quails and Korean quails, but only G loci in Beijing white quails. It was suggested that the AB genotype (or B allele) of Agrp exon I might be related to the black feather color of quails.
【作者单位】：河南科技大学动物科技学院;
【基金】：河南省产学研合作项目(152107000095.0) 河南科技大学标志性科技成果培育基金(2015BZCG02)
【分类号】：S839

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