小麦TaLEA1基因的表达特征及抗逆功能验证

发布时间：2018-03-29 09:26

本文选题：小麦　切入点：TaLEA　出处：《植物遗传资源学报》2017年02期

【摘要】：我国土壤盐碱化日益严重,对我国的粮食安全造成了严重威胁,因此耐盐基因挖掘对作物耐盐育种非常重要。许多研究表明胚胎发育晚期丰富蛋白(LEA)在植物应对非生物胁迫中发挥积极作用。本研究以小麦TaLEA1基因为研究对象,分析了其表达蛋白的理化性质及基因表达模式,并通过在拟南芥中过表达,分析Ta LEA1基因的抗逆功能。结果表明,TaLEA1基因的表达蛋白属于第3组LEA蛋白,是稳定的亲水蛋白,富含α-螺旋、β-转角等结构。Ta LEA1基因在小麦根、茎、叶、花、种子等不同组织中均有表达,盐胁迫条件诱导其高表达。在拟南芥中过表达TaLEA1基因,显著提高了盐胁迫下转基因拟南芥的种子萌发率、根长及盐和旱胁迫下的叶绿素含量。本研究结果为LEA基因抗逆机理的研究和耐盐基因的挖掘提供了重要信息。
[Abstract]:Soil salinization is becoming more and more serious in China, which poses a serious threat to the food security of our country. Therefore, salt tolerance gene mining is very important for crop salt tolerance breeding. Many studies have shown that late embryonic development rich protein Lea plays an active role in plant response to abiotic stress. This study focused on wheat TaLEA1 gene. The physicochemical properties and gene expression pattern of TaLEA1 protein were analyzed, and the stress resistance of Ta LEA1 gene was analyzed by overexpression in Arabidopsis thaliana. The results showed that the expressed protein of TaLEA1 gene belongs to the third group of LEA protein and is a stable hydrophilic protein. Ta LEA1 gene, which is rich in 伪 -helix and 尾 -corner, is expressed in different tissues of wheat, such as root, stem, leaf, flower, seed, etc. It is overexpressed in Arabidopsis thaliana under salt stress, and overexpressed in Arabidopsis thaliana. The seed germination rate, root length and chlorophyll content of transgenic Arabidopsis thaliana under salt stress were significantly increased. The results provided important information for the study of stress resistance mechanism of LEA gene and the excavation of salt tolerance gene.
【作者单位】：中国农业科学院作物科学研究所;山东师范大学生命科学学院;
【基金】：国家自然科学基金资助项目(31601302) 转基因植物新材料的育种价值评估(2016ZX08010-005) 中国农业科学院科技创新工程/作物种质资源鉴定与发掘团队
【分类号】：S512.1;Q943.2

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