不同休眠特性小麦中TaGAMyb-B基因的等位变异及其表达特性

发布时间：2018-03-29 21:31

本文选题：小麦　切入点：TaGAMyb-B　出处：《麦类作物学报》2016年06期

【摘要】：GAMyb基因编码蛋白作为GA信号途径的转录激活因子,对促进植物种子的萌发具有重要作用。为探讨GAMyb基因是否为导致小麦不同休眠特性的又一个原因,利用GAMyb基因在小麦3A、3B和3D染色体上的基因组序列设计了基因特异性引物,对不同休眠特性的白粒小麦中TaGAMyb-B基因的等位变异和转录本的表达特性进行了研究。结果表明,TaGAMyb-B基因在不同休眠特性的材料中存在SNPs和Indel的序列差异。根据不同休眠特性小麦材料TaGAMyb-B基因的序列差异,两个新的等位变异TaGAMyb-Ba和TaGAMyb-Bb被命名。进一步研究发现,两种不同等位变异的小麦材料在种子发育的不同时期TaGAMyb-B基因表达水平存在差异,说明TaGAMyb-B基因在不同休眠特性小麦材料中的等位变异影响了其表达。
[Abstract]:GAMyb gene encoding protein, as a transcriptional activator of GA signaling pathway, plays an important role in promoting the germination of plant seeds. The gene-specific primers were designed by using the genomic sequence of GAMyb gene on the 3Agn3B and 3D chromosomes of wheat. The allelic variation of TaGAMyb-B gene and the expression characteristics of transcripts in white grain wheat with different dormancy characteristics were studied. The results showed that there were differences between SNPs and Indel sequences in different dormancy materials. Sequence difference of TaGAMyb-B Gene in Sleeping characteristic Wheat, Two new allelic variants, TaGAMyb-Ba and TaGAMyb-Bb, were named. Further studies showed that there were differences in the expression levels of TaGAMyb-B gene between the two varieties at different stages of seed development. The results showed that allelic variation of TaGAMyb-B gene affected its expression in wheat with different dormancy characteristics.
【作者单位】：内蒙古农业大学生命科学学院植物生物技术功能实验室;
【基金】：国家自然科学基金项目(31260320,31160247) 内蒙古自治区自然科学基金项目(2014MS0338) 内蒙古农业大学优秀青年科学基金项目(2014XYQ-18)
【分类号】：S512.1

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