伽师瓜和86-1甜瓜几丁质酶基因的克隆与序列分析

发布时间：2018-03-30 08:50

本文选题：甜瓜　切入点：几丁质酶　出处：《保鲜与加工》2017年04期

【摘要】：以新疆主产不同耐藏性的甜瓜品种伽师瓜(耐藏性好)和86-1甜瓜(新密11号,耐藏性差)为试料,利用甜瓜基因组辅助设计引物克隆几丁质酶基因的c DNA序列,分别对其编码序列(CDS)及编码氨基酸进行相关生物信息学的预测分析,以期通过研究二者之间基因的差异揭示伽师瓜和86-1甜瓜采后不同耐藏性的机制。结果表明,获得的伽师瓜几丁质酶基因(Jia Shi Melon chitinase,JSMC2),NCBI检索号为KT921406;86-1甜瓜几丁质酶基因(Xin Mi-11 melon chitinase,XMMC),NCBI检索号为KU236388。JSMC2和XMMC序列长度均为1 011 bp,包含完整的开放阅读框,编码336个氨基酸,与黄瓜几丁质酶基因具有高度的同源性。蛋白结构域预测分析表明,甜瓜几丁质酶蛋白包含GH18_hevamine_Xip I_class_Ⅲ、Chi1、GH18_chitinase-like super family、Glyco-hydro-18和Glyco_18保守结构域,可催化几丁质水解成N-乙酰葡糖胺,分解真菌细胞的细胞壁,抵御病原真菌的侵染。
[Abstract]:Using Jiashi melon (good storage tolerance) and 86-1 melon (Xinmi11, poor storage tolerance) mainly produced in Xinjiang as the test materials, the c DNA sequence of chitinase gene was cloned by using genomic aided design primers of muskmelon. The related bioinformatics prediction and analysis of the coding sequence CDS) and the encoded amino acids were carried out respectively, in order to reveal the mechanism of different postharvest tolerance of Jiashi melon and 86-1 melon by studying the gene differences between them. The Shi Melon chitinase gene, Shi Melon chitinase, was obtained, and its search number was KT921406 / 86-1. The search numbers of KU236388.JSMC2 and XMMC sequences were both 1 011 BP and 1 011 BP, which contained a complete open reading frame and encoded 336 amino acids. The predicted protein domain analysis showed that the muskmelon chitinase protein contains GH18_hevamine_Xip I class3, Chi1GH18chitinase-like super family Glyco-hydro-18 and Glyco_18 conserved domains, which can catalyze the hydrolysis of chitinase-like super to N-acetylglucosamine, which can catalyze the hydrolysis of chitinase to N-acetylglucosamine. The cell wall of fungal cells is broken down to resist the infection of pathogenic fungi.
【作者单位】：新疆农业大学食品科学与药学学院;
【基金】：新疆农业大学校前期资助项目(XJAU201421)
【分类号】：S652

【参考文献】