PTEN和EMMPRIN在肺腺癌组织中的表达及其与EGFR基因突变的关系

发布时间：2018-03-30 15:38

本文选题：肺腺癌　切入点：PTEN　出处：《肿瘤防治研究》2017年05期

【摘要】：目的观察肺腺癌中PTEN和EMMPRIN蛋白水平的表达,分析其与肺腺癌临床病理特征及EGFR基因突变的关系。方法选取10例正常肺组织作为对照组,分别用免疫组织化学法和PCR法检测90例肺腺癌组织中PTEN和EMMPRIN蛋白的表达和EGFR基因突变情况。结果肺腺癌组织中PTEN蛋白的表达显著低于正常肺组织,EMMPRIN蛋白的表达显著高于正常肺组织。PTEN和EMMPRIN表达率为40%(36/90)和80%(72/90),EGFR基因突变率为47.78%(43/90)。PTEN的表达与肺腺癌组织学类型有关(P=0.000),EMMPRIN的表达与淋巴结转移状况及TNM分期有关(均P0.05)。Spearman相关分析显示PTEN和EMMPRIN呈显著负相关(rp=-0.215,P=0.041),PTEN与EGFR基因突变呈正相关(rp=0.490,P=0.000),EMMPRIN与EGFR基因突变无明显相关性(rp=0.170,P=0.110)。结论肺腺癌中PTEN和EMMPRIN蛋白可能存在某些机制共同作用促进了肺腺癌的发生发展,PTEN蛋白的表达与EGFR基因突变呈正相关。
[Abstract]:Objective to observe the expression of PTEN and EMMPRIN protein in lung adenocarcinoma, and to analyze the relationship between the expression of PTEN and EMMPRIN protein and the clinicopathological characteristics of lung adenocarcinoma and the mutation of EGFR gene. Methods Ten normal lung tissues were selected as control group. Immunohistochemical method and PCR method were used to detect the expression of PTEN and EMMPRIN protein and EGFR gene mutation in 90 cases of lung adenocarcinoma. Results the expression of PTEN protein in lung adenocarcinoma was significantly lower than that in normal lung tissue. The expression of EMMPRIN was higher than that of normal lung tissue (40 / 36 / 90) and 80 / 72 / 90 / 90 (47.78%(43/90).PTEN). The expression of EMMPRIN was related to the histological type of lung adenocarcinoma. The expression of EMMPRIN was related to lymph node metastasis and TNM stage (all P0.05).Spearman correlation analysis showed that PTEN and EMMPRIN were present.). There was significant negative correlation between EGFR gene mutation and PTEN gene mutation. There was no significant correlation between PTEN and EGFR gene mutation. Conclusion there may be some mechanisms of PTEN and EMMPRIN protein in lung adenocarcinoma to promote the expression of PTEN protein in the pathogenesis and development of lung adenocarcinoma. There was a positive correlation with the mutation of EGFR gene.
【作者单位】：郑州人民医院病理科;郑州人民医院院士工作站;
【基金】：郑州市普通科技攻关计划(20150076)
【分类号】：R734.2

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