不同来源的副溶血性弧菌毒素基因检测和耐药性分析

发布时间：2018-03-30 21:31

本文选题：副溶血性弧菌　切入点：毒素基因　出处：《江苏预防医学》2016年06期

【摘要】：目的了解常熟地区副溶血性弧菌中毒素基因的分布和耐药性情况,为指导用药提供依据。方法利用实时荧光PCR方法,对常熟地区2013—2014年分离的211株副溶血性弧菌进行毒素基因检测;对2014年分离的134株副溶血性弧菌进行耐药性分析。结果在211株副溶血性弧菌中,检测出毒素基因阳性98株,占46.4%。其中,来源为食物中毒的菌株60株,检测出毒素基因阳性41株(68.3%);来源为食源性疾病哨点监测的菌株74株,检测出毒素基因阳性57株(77.0%);来源于环境水或水产品的菌株77株,未检测到毒素基因。20种药物的耐药性检测发现,97.76%的副溶血性弧菌对氨苄西林耐药,部分菌株对头孢呋辛、头孢噻吩、阿米卡星耐药,少数菌株对四环素、庆大霉素和复方新诺明耐药,对其他抗生素均敏感。结论不同来源的副溶血性弧菌菌株毒素基因阳性比例有显著差异;除氨苄西林外,其他抗生素对副溶血性弧菌导致的腹泻有部分或较好的疗效。
[Abstract]:Objective to investigate the distribution and drug resistance of toxin gene in Vibrio parahaemolyticus in Changshu, and to provide the basis for guiding drug use. The toxin genes of 211 strains of Vibrio parahaemolyticus isolated in Changshu from 2013 to 2014 were detected, and drug resistance of 134 strains of Vibrio parahaemolyticus isolated in 2014 were analyzed. Results among 211 strains of Vibrio parahaemolyticus, 98 strains of vibrio parahaemolyticus were detected positive for toxin gene. Among them, 60 strains were from food poisoning, 41 strains were positive for toxin gene, 74 strains were from sentinel surveillance of foodborne diseases, 57 strains were positive for toxin gene, 77 strains were from environmental water or aquatic products, No toxin gene was detected. 20 drug resistance tests showed that 97.76% of Vibrio parahaemolyticus were resistant to ampicillin, some strains were resistant to cefuroxime, cefothiophene, amikacin, and a few strains were tetracycline. Both gentamicin and compound sulfamide were sensitive to other antibiotics. Conclusion the positive rate of toxin gene of Vibrio parahaemolyticus strains from different sources was significantly different, except ampicillin, ampicillin, ampicillin, ampicillin, ampicillin, ampicillin, ampicillin, Other antibiotics have partial or better effects on diarrhea caused by Vibrio parahaemolyticus.
【作者单位】：江苏省常熟市疾病预防控制中心;
【分类号】：R440

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