黄腐酸对Haematococcus pluvialis LUGU虾青素积累和lcy基因表达量的影响

发布时间：2018-03-31 02:28

本文选题：黄腐酸　切入点：诱导　出处：《食品与生物技术学报》2017年07期

【摘要】：雨生红球藻是虾青素的主要来源,为探明黄腐酸(FA)对雨生红球藻Haematococcus pluvialis LUGU的影响,将不同质量浓度的FA添加至对数生长期的藻液中置于胁迫条件下培养,并分别对细胞生物量浓度、虾青素积累质量浓度以及番茄红素β-环化酶(lcy)基因表达量进行了测定。结果表明,黄腐酸质量浓度为10 mg/L,藻细胞的生物量产率达到最大80.28 mg/(L·d),比对照组提高5.44%;而虾青素最大质量浓度为20.82 mg/L,是在FA质量浓度为5 mg/L时测得,比对照组提高了86.89%。RT-PCR分析显示,虾青素合成的关键基因lcy的表达受FA的影响,当添加5 mg/L和10 mg/L的黄腐酸时lcy基因最大的表达量分别为对照的1.2倍和0.7倍。实验证明FA诱导下的雨生红球藻虾青素的积累含量和lcy基因表达量呈正相关。
[Abstract]:The main source of astaxanthin was Haematococcus pluvialis. In order to find out the effect of fulvic acid on Haematococcus pluvialis LUGU, different concentrations of FA were added to the algal solution of logarithmic growth period and cultured under stress.Cell biomass concentration, astaxanthin accumulation concentration and lycopene 尾 -cyclase gene expression were determined.The results showed that when the concentration of fulvic acid was 10 mg / L, the biological yield of algal cells reached the maximum of 80.28 mg/(L DX, which was 5.4445% higher than that of the control group, while the maximum mass concentration of astaxanthin was 20.82 mg / L at 5 mg/L, which was higher than that in the control group by 86.89%.RT-PCR analysis.The expression of lcy, the key gene of astaxanthin synthesis, was affected by FA. The maximum expression of lcy gene was 1.2 times and 0.7 times as much as that of the control when 5 mg/L and 10 mg/L fulvic acid were added, respectively.The results showed that the accumulation of astaxanthin induced by FA was positively correlated with the expression of lcy gene.
【作者单位】：昆明理工大学生命科学与技术学院;
【基金】：国家自然科学基金项目(21266013)
【分类号】：Q949.2

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