三个胡杨SnRK基因的克隆和序列分析

发布时间：2018-04-01 03:04

本文选题：SnRK　切入点：基因克隆　出处：《分子植物育种》2017年09期

【摘要】：蔗糖非依赖1蛋白激酶(sucrose non-fermenting-1-related protein kinase,Sn RK)是广泛存在于植物中的一类Ser/Thr蛋白激酶,参与各种胁迫信号传导通路,对植物抵御不良环境起到重要作用。本研究根据胡杨转录组测序结果克隆出了三条Sn RK基因,进行序列对比和系统进化分析预测其基因功能。结果显示这三个胡杨Sn RK基因属于Sn RK2家族成员,三个胡杨Sn RK基因又与AtSnRK2.2、AtSnRK2.3、AtSnRK2.6序列相似性较高,可能在逆境应答过程中具有重要功能。胡杨Sn RK的功能预测和分析,将为进一步研究林木非生物逆境胁迫响应机制奠定基础。
[Abstract]:Sucrose non-fermenting-1-related protein kinaseSn-RK is a kind of Ser/Thr protein kinase widely found in plants, which participates in various stress signal transduction pathways and plays an important role in plant resistance to adverse environment.In this study, three Sn RK genes were cloned according to the results of transcriptome sequencing of Populus euphratica, and their gene functions were predicted by sequence comparison and phylogenetic analysis.The results showed that these three poplar Sn RK genes belong to the Sn RK2 family, and the three poplar Sn RK genes are similar to AtSnRK2.2 and AtSnRK2.3 / AtSnRK2.6 sequences, which may play an important role in the process of stress response.The functional prediction and analysis of Sn RK of Populus euphratica will lay a foundation for further study on the response mechanism of abiotic tree to stress.
【作者单位】：北京林业大学生物科学与技术学院林木育种国家工程实验室;
【基金】：国家自然科学基金面上项目(31370597;31670610)资助
【分类号】：Q943.2;S792.11

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