鸭MITF、KIT基因SNP与屠宰、肉质性状的关联分析

发布时间：2018-04-01 01:02

本文选题：鸭　切入点：MITF基因　出处：《华中农业大学》2017年硕士论文

【摘要】：MITF基因编码小眼畸形相关转录因子,KIT基因编码干细胞因子受体,多数文献是对MITF基因、KIT基因在黑色素形成中起的重要作用及它们与动物毛色之间的关联进行的研究。MITF基因可调控PGC-1α(过氧化物酶体增值剂受体γ共激活剂1-α),PGC-1α能够影响肌肉功能,如肌肉耐力,肌纤维类型转换。该基因能够与MAPK1互相作用,而MAPK信号途径的活化可以进一步增加骨骼肌纤维细胞蛋白含量,增加肌纤维长度和横截面直径,据此推测MITF基因对肌肉的生长发育有一定的影响,而KIT基因是MITF基因的靶基因,并且可调控骨髓造血干细胞HSC,对于血细胞的正常生成具有重要作用,血细胞中的红细胞可为肌细胞供氧,对于肌细胞的生长发育有非常重要的作用,因此推测KIT基因对肌肉的生长发育也有一定的影响,肌肉的生长发育情况会直接影响家禽的屠宰、肉质性能的优劣,故推测MITF基因与KIT基因会在一定程度上影响家禽的屠宰、肉质性能,但国内外对这两个基因与动物屠宰、肉质性状之间的关联研究鲜少报道,所以本实验使用实验室现存的连城白鸭和白改鸭杂交后建立的F2代资源群体以及樱桃谷鸭作为实验样本,对MITF基因的11个SNPs位点和KIT基因的8个SNPs位点进行PCR-RFLP检测,统计基因分型结果,并与鸭屠宰及肉质性状进行关联分析,探讨MITF、KIT基因作为影响鸭屠宰及肉质性状候选基因的可能性,主要研究结果如下:1.对MITF基因和KIT基因在鸭肌肉等组织中进行表达谱分析,结果发现MITF和KIT基因在鸭心、肝、脾、肺、肾、胃、腿肌、胸肌中都有表达;2.利用RNA-Seq的相关数据,发现了MITF基因90多个疑似SNPs,利用其中11个SNPs进行酶切分型,其基因型与鸭屠宰、肉质性状进行关联分析发现:(1)与鸭肉质性状相关的SNP位点如下:与鸭腿肌纤维直径相关的位点是C808002T、C808890T、C820188T、T844468C和G846879A位点;与鸭腿肌或胸肌肉色相关的位点是C808890T、T810554G、C820188T、G834108T和T844865C位点;与鸭胸肌或腿肌pH相关的位点是G810251T、C810282T和G834108T位点;与鸭胸肌或腿肌失水率相关的位点是G810251T、C810282T、C810754A、G834108T和T844865C位点;与鸭胸肌或腿肌熟肉率相关的位点是C810754A和G834108T位点;与鸭腿肌或胸肌破碎系数相关的位点是G834108T和T844468C位点;(2)与鸭屠宰性状相关的SNP位点如下:与鸭屠宰率相关的位点是C810754A和G834108T位点;与鸭心脏指数相关的位点是T810554G和T844468C位点;与鸭肝重、肝脏指数、腹脂皮脂相关的位点是C810754A位点;3.对本实验室前期利用混池测序在鸭KIT基因中发现的SNP,在资源群体中进行了酶切分型,对基因型与鸭屠宰、肉质性状进行关联分析发现:(1)与鸭肉质性状相关的SNP位点如下:与鸭腿肌或胸肌肉色相关的位点是T5168C、A1238C、G1064C、A6578G、A1221T、A5557G和C1243T位点;与鸭胸肌或腿肌失水率相关的位点是G1064C位点;与鸭胸肌熟肉率相关的位点是A6578G位点;与胸肌破碎系数相关的位点是T5168C和A1221T位点;(2)与鸭屠宰性状相关的SNP位点如下:与鸭屠宰率相关的位点是T5032C和A1221T位点;与鸭心脏指数相关的位点是G1064C位点;与鸭腹脂或皮脂相关的位点是T5168C、A1221T、A5557G和C1243T位点;综上所述:MITF基因、KIT基因的SNP与鸭屠宰、肉质性状相关,推测MITF基因、KIT基因可作为影响鸭屠宰及肉质性状的候选基因。
[Abstract]:The MITF gene encoding microphthalmia associated transcription factor, KIT gene encoding stem cell factor receptor, most of the literature is on the MITF gene, KIT gene in melanin formation of.MITF gene play an important role in the association between them and the color of the animal and the regulation of PGC-1 alpha (peroxidase body added receptor gamma CO activation agent 1-), alpha PGC-1 alpha can affect muscle function, such as muscle endurance, muscle fiber type conversion. The gene can interact with MAPK1, and the activation of MAPK signaling pathway can increase the protein content of skeletal muscle fibers, increase muscle fiber length and diameter of cross section, it is inferred that MITF gene has a certain influence and development for muscle growth, while the KIT gene is the target gene of MITF gene, and the regulation of bone marrow hematopoietic stem cells HSC, plays an important role in the normal production of blood cells, blood cells red blood cells for Muscle cells for oxygen, plays a very important role in muscle cell growth and development, so it has certain effects on the growth of KIT gene on muscle development, growth and development of muscle will directly affect poultry slaughtering, meat performance, suggesting that MITF gene and KIT gene can influence the slaughter of poultry in some degree meat performance, but at home and abroad on the two genes and animal slaughter, rarely reported in association studies between meat quality traits, so this experiment use the existing laboratory of Liancheng white duck and white duck changed after the establishment of the hybrid F2 generation resource group and cherry valley duck as the experimental samples, 8 SNPs loci and 11 SNPs loci and KIT the gene of MITF gene detected by PCR-RFLP genotyping results, statistics, and analysis, explore the association of MITF with duck slaughter and meat quality traits, KIT gene as the effects of duck slaughtering and meat quality trait candidate The possibility of genetic, the main results are as follows: 1. the MITF gene and KIT gene in duck muscle expression spectrum analysis, the results showed that MITF and KIT genes in duck heart, liver, spleen, lung, kidney, stomach, leg muscle, breast muscle are expressed; 2. using RNA-Seq data. MITF gene was found more than 90 suspected SNPs, using the 11 SNPs RFLP, the genotype and the duck slaughtering, meat quality traits correlation analysis found that: (1) SNP locus associated with duck meat quality traits as follows: associated with duck leg muscle fiber diameter is C808890T, C820188T loci C808002T, T844468C, and G846879A locus; associated with duck leg muscle and breast muscle color loci C808890T, T810554G, C820188T, G834108T and T844865C loci; associated with duck breast or leg muscle pH sites are G810251T, C810282T and G834108T loci; associated with duck breast or leg muscle water loss rate of site is G810251T, C8 10282T, C810754A, G834108T and T844865C loci; associated with duck breast or leg muscle rate of cooked meat loci is C810754A and G834108T sites; and duck leg muscle or muscle breaking coefficient related site is G834108T and T844468C loci; (2) SNP locus associated with duck carcass traits are as follows: the rate of related sites and duck slaughter is the C810754A and G834108T loci; associated with duck heart index is T810554G loci and T844468C loci; and the liver weight, liver index, abdominal fat sebum related site is C810754A site; 3. of the previous use of mixed pool sequencing in duck KIT gene found in SNP, the RFLP in resource group. The genotypes with duck slaughtering, meat quality traits correlation analysis found that: (1) SNP locus associated with duck meat quality traits as follows: associated with duck leg muscle and breast muscle color loci T5168C, A1238C, G1064C, A6578G, A1221T, A5557G and C1243T Point; associated with duck breast or leg muscle water loss rate of loci is G1064C loci; rate related loci and A6578G loci is the duck breast meat; related sites and chest breaking coefficient is T5168C and A1221T loci; (2) SNP locus associated with duck carcass traits are as follows: the rate of related sites and duck slaughter is T5032C and A1221T sites; associated with duck heart index locus is associated with the G1064C locus; duck abdominal fat or sebum sites are T5168C, A1221T, A5557G and C1243T loci; Summary: MITF gene, KIT gene and SNP duck slaughtering, meat quality traits, suggesting that MITF gene, KIT gene can be used as a candidate gene of duck slaughter and meat quality traits.

【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S834

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