盐胁迫下卷丹百合MAPK基因的克隆和序列分析

发布时间：2018-03-31 22:33

本文选题：卷丹百合　切入点：促分裂原活化蛋白激酶(MAPK)　出处：《分子植物育种》2016年09期

【摘要】：本研究通过卷丹百合在不同浓度的盐胁迫下确定了盐浓度阈值,利用同源克隆和RACE技术获得了盐阈值浓度处理下卷丹百合MAPK基因的保守区(Genbank登录号:JQ437268),并对其进行生物信息学分析。同时利用了DNAman软件和Blastn序列比对在基因和蛋白角度分析了MAPK基因在盐阈值浓度处理下的表达情况。分析表明,卷丹百合盐阈值浓度为1.09 mg/m L,MAPK基因保守区长627 bp,推测编码209个氨基酸,有1个高度保守的区域(TRWYRAPE)存在于MAPK氨基酸序列中,通过NCBI上的BLAST比对分析,发现这段保守区序列为促分裂原活化蛋白激酶超家族的催化活性区。
[Abstract]:In this study, the threshold of salt concentration was determined under different concentrations of salt stress. By using homologous cloning and RACE technique, the conserved region of MAPK gene of Lilium danlifolia was obtained by using the salt threshold concentration treatment. The accession number of Genbank was: JQ437268, and its bioinformatics analysis was carried out. Meanwhile, DNAman software and Blastn sequence alignment were used in gene and protein alignment. The expression of MAPK gene under salt threshold concentration was analyzed in this paper. The threshold concentration of the salt in lily was 1.09 mg/m, the conserved region of the MAPK gene was 627 BP, which was deduced to encode 209 amino acids, and a highly conserved region, TRWYRAPE, existed in the amino acid sequence of MAPK. The sequence was analyzed by BLAST comparison on NCBI. The conserved region was found to be the catalytic active region of the mitogen-activated protein kinase superfamily.
【作者单位】：沈阳农业大学辽宁省农业生物技术重点实验室设施园艺省部共建教育部重点实验室;
【基金】：国家自然基金(31572150) 高等学校博士学科点专项科研基金课题(20112103120005)共同资助
【分类号】：S682.29

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