大丽轮枝菌黑色素合成相关基因与微菌核形成的关系

发布时间：2018-04-01 05:13

本文选题：DHN黑色素　切入点：Vayg　出处：《菌物学报》2017年12期

【摘要】：大丽轮枝菌是一种重要的土传植物病原真菌,以休眠结构微菌核作为初始接种体,可侵染660多种植物引致黄萎病。微菌核是致密的多细胞结构,表面附着大量的DHN黑色素。许多报道指出,在微菌核发育过程中,传统的DHN黑色素合成途径中有5种催化酶编码基因Vd PKS、Vd T4HR、Vd SCD、Vd T3HR和Vd LAC均被诱导表达,但这些基因与微菌核形成的关系目前尚无报道。本研究通过基因敲除技术,系统研究了传统DHN黑色素合成通路上这5种关键酶编码基因及一种缩链催化酶编码基因Vayg1在大丽轮枝菌黑色素合成及微菌核形成中的作用。结果表明,大丽轮枝菌DHN黑色素合成需要Vayg1基因的参与,且Vayg1和Vd T3HR基因还参与微菌核的形成过程。因此,Vayg1基因和Vd T3HR基因可作为黄萎病防治的新靶标。
[Abstract]:Verticillium dahliae is an important soil borne plant pathogenic fungi, dormant microsclerotia structure as the initial inoculum, which can infect more than 660 species of plants caused by Verticillium wilt. Microsclerotia is a multicellular structure compact, a large number of DHN melanocytes attached to the surface. Many reports pointed out that in the process of microsclerotia development, there are 5 kinds of catalyst the enzyme Vd gene encoding PKS, DHN melanin biosynthesis pathway in the traditional Vd T4HR, Vd SCD, Vd T3HR and Vd LAC were induced, but the relationship between these genes and the microsclerotia formation has not been reported. This study by gene knockout technology, studied the synthetic pathway of melanin on the 5 traditional DHN the key enzyme gene encoding and a role of shrink chain catalytic enzyme encoding gene Vayg1 in Verticillium dahliae microsclerotia and melanin synthesis in the formation. The results show that Verticillium dahliae DHN melanin synthesis needs the participation of Vayg1 gene, and Vayg1 and Vd T3H The R gene is also involved in the formation of microsclerotium. Therefore, the Vayg1 gene and the Vd T3HR gene can be used as a new target for the prevention and control of Verticillium wilt.

【作者单位】：西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室;中国农业科学院棉花研究所;
【基金】：国家自然科学基金(31371888)~~
【分类号】：S435.621.2

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