南瓜肌醇单磷酸酶基因CmIMP1和CmIMP2的克隆与表达分析

发布时间：2018-04-02 00:08

  本文选题：南瓜　切入点：CmIMP　出处：《园艺学报》2016年07期

【摘要】：以中国南瓜(Cucurbita moschata)为试验材料,利用同源克隆和南瓜转录组unigene序列获得2个IMP基因的全长c DNA序列,将两个基因命名为Cm IMP1和Cm IMP2,Gen Bank登录号为KP735607和KP735608。Cm IMP1基因c DNA全长1 053 bp,包含1个810 bp的ORF,共编码269个氨基酸;Cm IMP2基因c DNA全长945 bp,包含1个807 bp的ORF,共编码268个氨基酸。序列分析发现两个基因均含有磷酸酶家族锂敏感的3个特殊结构域,系统进化树分析表明这2个南瓜Cm IMP与其他植物IMP有较高同源性(63.8%~94.0%),并与葫芦科作物最接近。采用荧光定量PCR研究Cm IMP在各组织及逆境条件下的表达模式显示,其表达具有组织特异性,在叶中表达最高,须和幼果次之;盐胁迫和干旱胁迫可强烈诱导Cm IMP表达,ABA对Cm IMP的诱导较微弱,推测Cm IMP在南瓜响应非生物胁迫的分子调控机制方面发挥重要作用。
[Abstract]:Using Cucurbita moschata (Cucurbita moschata) as experimental material, two full-length c DNA sequences of IMP gene were obtained by homologous cloning and unigene sequence of pumpkin transcriptome.Sequence analysis showed that the two genes contained three special domains sensitive to lithium phosphatase family. Phylogenetic tree analysis showed that the two pumpkin cm IMP had a high homology with other plant IMP, 63.80.It was the closest to Cucurbitaceae crops.Fluorescence quantitative PCR was used to study the expression patterns of cm IMP in different tissues and under different stress conditions. The results showed that the expression of cm IMP was tissue specific, the highest in leaves, and the second in young fruits.Salt stress and drought stress could strongly induce the expression of cm IMP on cm IMP, suggesting that cm IMP plays an important role in the molecular regulation of pumpkin response to abiotic stress.
【作者单位】： 暨南大学生命科学技术学院;广东省农业科学院蔬菜研究所;华中农业大学园艺林学学院;广东省蔬菜新技术研究重点实验室;
【基金】：广东省自然科学基金项目(2015A030310133) 广东省省级科技计划项目(2015A030302044,2015B070701014) 广东省农业科学院院长基金项目(201403)
【分类号】：S642.1
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本文编号：1697895