结缕草ZjNAC基因的克隆与表达分析

发布时间：2018-04-02 01:02

本文选题：结缕草　切入点：NAC转录因子　出处：《草业学报》2016年04期

【摘要】：NAC类家族的基因是植物特有的转录因子,并且它在植物的生长发育中发挥着重要的作用。本研究以结缕草为研究材料,对结缕草转录组数据库利用local blast的办法克隆出1个NAC类转录因子。为深入探讨和研究NAC类转录因子在植物逆境生理上的作用奠定基础。通过与水稻的OsNACs家族成员进行进化树分析,结合蛋白分析,QRT-PCR等的方式探索其生物学功能。实验结果表明,该基因长度为1496bp,包含1个完整开放阅读框(ORF),长度为1332bp,编码449个氨基酸,该蛋白含有1个NAM结构域。与水稻的NAC类转录因子家族的系统进化树对比发现,它与OsNAC036近缘性最高,因此将该基因命名为ZjNAC036。QRT-PCR结果显示,该基因在干旱情况下表达量呈上升趋势,并且干旱处理8h的时候,差异倍数最大,达到了50倍左右,而在盐胁迫、低温胁迫下没有规律性变化。这表明ZjNAC036在干旱逆境生理上可能有重要的作用,而在高盐及寒冷的逆境生理方面可能作用不大。
[Abstract]:NAC family genes are plant specific transcription factors and play an important role in plant growth and development. A NAC transcription factor was cloned from Zoysia japonica transcriptome database by local blast method, which laid a foundation for further study on the role of NAC transcription factors in plant stress physiology. For evolutionary tree analysis, The results showed that the gene was 1496 BP in length and contained a complete open reading frame, ORF, with a length of 1332 BP, encoding 449 amino acids. Compared with the phylogenetic tree of NAC transcription factor family of rice, the protein has the highest affinity to OsNAC036. Therefore, the result of naming the gene ZjNAC036.QRT-PCR shows that the expression of this gene is on the rise under drought. After 8 hours of drought treatment, the difference was about 50 times, but there was no regular change under salt stress and low temperature stress, which indicated that ZjNAC036 might play an important role in drought stress physiology. But it may have little effect on the physiology of high salt and cold stress.
【作者单位】：北京林业大学草坪研究所;河北省作物生长调控重点实验室河北农业大学;
【基金】：中央高校基本科研业务费专项资金(BLX2012034) 国家高新技术研究发展计划项目(2013AA102607)资助
【分类号】：S688.4

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