牡丹开花调控转录因子基因PsFUL1的克隆与表达分析

发布时间：2018-04-03 01:31

  本文选题：牡丹　切入点：PsFUL　出处：《植物生理学报》2017年04期

【摘要】：以‘洛阳红’牡丹为试验材料,采用RT-PCR方法从其花芽中克隆得到1个开花调控重要转录因子基因FRUITFULL(FUL1)的同源基因,其c DNA开放阅读框长度为768 bp,编码255个氨基酸。序列比对和结构域分析表明,此蛋白具有MADS超家族、K-box超家族的结构域和ARG80多结构域,且具有MADS-box和K-box的稳定保守基序。NCBI blast分析发现克隆得到的氨基酸序列与葡萄中的FUL类蛋白具有较高同源性,为69%。因此将其命名为Ps FUL1,Gen Bank登录号为KX621277。系统进化树分析表明,Ps FUL1与葡萄编码的Vv FUL1亲缘关系最近,属于MADS基因家族中的AP1/FUL亚家族。q RT-PCR表明,Ps FUL1基因在‘洛阳红’牡丹不同组织中表达差异显著,在花芽和花瓣中的表达量最高,苞片和叶片次之,根中最少。在不同品种牡丹(早花‘迎日红’、中花‘洛阳红’、晚花‘花王’)的6个不同开花时期的花瓣中,Ps FUL1基因表达量因花期早晚与开花时期不同而差异显著,表明Ps FUL1基因对牡丹开花及花期的早晚有重要调控作用。
[Abstract]:A homologous gene of FRUITFULL FUL1, an important transcription factor gene, was cloned from the flower bud of 'Luoyang Red' peony by RT-PCR method. Its c DNA open reading frame length was 768 BP, encoding 255 amino acids.Sequence alignment and domain analysis showed that the protein had the domain of MADS superfamily K-box superfamily and ARG80 multi-domain.The stable conserved motif of MADS-box and K-box. NCBI blast analysis showed that the cloned amino acid sequence had high homology with the FUL class protein in grape.Therefore, it is named as PS FUL1G Bank login number is KX621277.Phylogenetic tree analysis showed that the phylogenetic relationship between PPS FUL1 and VV FUL1 encoded by grape was the most recent. AP1/FUL subfamily. Q RT-PCR, belonging to MADS gene family, showed significant difference in expression of PPS FUL1 gene in different tissues of 'Luoyang Red' peony.The highest expression was found in flower buds and petals, followed by bracts and leaves, and the least in roots.The expression of Ps FUL1 gene in the petals of different peony cultivars (early flower 'Yingri', midflower 'Luoyang' Hongwei, late flower 'Wang') in 6 different flowering stages was significantly different from that in flowering time, and that in early and late flowering stage, there was a significant difference in the expression of Ps FUL1 gene in the petal of the petal in different flowering stage.The results indicated that Ps FUL1 gene played an important role in regulating the flowering and flowering of peony.
【作者单位】： 河南科技大学农学院/牡丹学院;河南省油用牡丹工程技术研究中心;河南科技大学林学院;
【基金】：河南省高校科技创新团队支持计划(14IRTSTHN014) 国家自然科学基金(31370697) 河南省科技创新杰出人才基金(164200510013)~~
【分类号】：Q943.2;S685.11
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本文编号：1703041