TERT基因转染BMSC血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响

发布时间：2018-04-03 02:03

本文选题：端粒酶逆转录酶　切入点：骨髓基质干细胞　出处：《重庆医学》2017年10期

【摘要】：目的探讨端粒酶逆转录酶(TERT)基因转染骨髓基质干细胞(BMSC)对血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响。方法 60只大鼠随机分为阴性对照组(A组)、模型组(B组)、常规BMSC组(C组)和转染BMSC组(D组);采用Morris迷宫测试、RT-PCR和蛋白免疫印迹法检测各组大鼠相关指标。结果 C组、D组逃避潜伏期显著长于B组;D组逃避潜伏期显著长于B组。与A组比较,B组、C组、D组脑源性神经营养因子(BDNF)mRNA、TERT mRNA、突触前区突触素(SYP)mRNA及蛋白表达均显著降低,A组大鼠突触间隙排列清晰,SYN阳性细胞数较多;D组突触间隙更为清晰,SYN阳性细胞数量和突出间隙排列结构都接近于A组。结论 TERT转染BMSC对血管性痴呆大鼠的治疗作用明显,其作用机制与促进BDNF、TrkB表达、改善突触的可塑性有关。
[Abstract]:Objective to investigate the telomerase reverse transcriptase (TERT) gene transfected bone marrow stromal cells (BMSC) influence on memory function and synaptic plasticity of hippocampal CA1 area of vascular dementia rats. Methods 60 rats were randomly divided into negative control group (A group), model group (B group), BMSC group (conventional group C) and BMSC group (group D); using Morris maze test, rats were detected the indexes related to RT-PCR and Western blot. Results C group, D group, the escape latency was significantly longer than that of B group; group D, the escape latency was significantly longer than that of B group. Compared with A group, B group, C group, brain D group of neurotrophic factor (BDNF) mRNA, TERT mRNA, presynaptic synaptophysin (SYP) expression of mRNA and protein were significantly decreased, A rats with a clear synaptic gap, the number of SYN positive cells in D group are more; the synaptic gap is more clear, the number of SYN positive cells and prominent gap structure are close to the A group conclusion TERT BMSC transfection on. The therapeutic effect of vascular dementia rats is obvious. Its mechanism is related to promoting the expression of BDNF and TrkB and improving the plasticity of synapse.

【作者单位】：张家口学院医学院;解放军251医院重症医学科;河北医科大学第二医院神经内科;
【基金】：国家自然科学基金资助项目(81171210)
【分类号】：R749.13

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