河南省16个主栽小麦品种抗叶锈基因分析

发布时间：2018-04-03 09:45

本文选题：小麦　切入点：叶锈病　出处：《植物遗传资源学报》2017年03期

【摘要】：为了明确河南省小麦品种的抗叶锈性及抗叶锈基因的分布,为小麦品种推广与合理布局、叶锈病防治及抗病育种提供依据,本研究利用2015年采自河南省的5个小麦叶锈菌流行小种混合菌株,对近几年河南省16个主栽小麦品种进行了苗期抗性鉴定,然后选用12个小麦叶锈菌生理小种对这些品种进行苗期基因推导,同时利用与24个小麦抗叶锈基因紧密连锁(或共分离)的30个分子标记对该16个品种进行了抗叶锈基因分子检测。结果显示,供试品种苗期对小麦叶锈菌混合流行小种均表现高度感病;基因推导与分子检测结果表明,供试品种可能含有Lr1、Lr16、Lr26和Lr30这4个抗叶锈基因,其中先麦8号含有Lr1和Lr26;郑麦366和郑麦9023含有Lr1;西农979和怀川916含有Lr16;中麦895、偃展4110、郑麦7698、平安8号、众麦1号、周麦16、衡观35和矮抗58含有Lr26;周麦22中含有Lr26,还可能含有Lr1和Lr30;豫麦49-198和洛麦23可能含有本研究中检测以外的其他抗叶锈基因。因此,河南省主栽小麦品种的抗叶锈基因丰富度较低,今后育种工作应注重引入其他抗叶锈性基因,提高抗叶锈性,有效控制小麦叶锈病。
[Abstract]:In order to clarify the leaf rust resistance and the distribution of leaf rust resistance genes of wheat varieties in Henan Province, it is necessary to provide the basis for the popularization and rational distribution of wheat varieties, the prevention and control of leaf rust and breeding for disease resistance.In this study, the seedling resistance of 16 main wheat varieties in Henan Province in recent years was identified by using 5 hybrid wheat leaf rust strains collected from Henan Province in 2015.Then 12 physiological races of wheat leaf rust were selected to deduce the genes of these varieties at seedling stage.At the same time, the leaf rust resistance genes of 16 wheat varieties were detected by 30 molecular markers closely linked (or coisolated) with 24 wheat leaf rust resistance genes.The results showed that the cultivars were highly susceptible to mixed endemic races of wheat leaf rust at seedling stage, and the results of gene derivation and molecular detection showed that the tested varieties might contain four leaf rust resistant genes, Lr1, LR16, Lr26 and Lr30, respectively.Among them, Shenmai 8 contains Lr1 and Lr26; Zhengmai 366 and Zhengmai 9023 contain Lr1; Xinong 979 and Huichuan 916 contain Lr16; Zhongmai 895, Yan Zhan 4110, Zheng Mai 7698, Ping an 8, Zhongmai 1,Zhoumai 16, Hengguang 35 and dwarf resistance 58 contained Lr26, Zhoumai 22 contained Lr26, possibly Lr1 and Lr30, and Yumai 49-198 and Lomai 23 may contain other leaf rust resistance genes other than those detected in this study.Therefore, the abundance of leaf rust resistance genes of main wheat varieties in Henan Province is low. In the future breeding work should focus on introducing other leaf rust resistance genes to improve leaf rust resistance and effectively control wheat leaf rust.
【作者单位】：河北农业大学植物保护学院/国家北方山区农业工程技术研究中心/河北省农作物病虫害生物防治工程技术研究中心;
【基金】：国家重点基础研究发展计划(2013CB127700) 河北省自然科学基金项目(C2015204105)
【分类号】：S512.1

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