杜仲愈伤组织中类胡萝卜素合成相关基因的表达分析

发布时间：2018-04-04 02:24

  本文选题：杜仲　切入点：愈伤组织　出处：《分子植物育种》2017年01期

【摘要】：通过对杜仲愈伤组织转录组高通量测序结果进行KEGG通路深入分析发现,强光(光强为12 000 Lx,16 h光照,8 h黑暗)培养杜仲愈伤组织18 d,黑暗培养作为对照,与类胡萝卜素合成相关的七种酶(九个基因)上调表达,七种酶分别为9-顺式-环氧类胡萝卜素双加氧酶(EC 1.13.11.51,9-cis-epoxycarotenoid dioxygenase),脱落酸8-羟化酶(EC 1.14.13.93,(+)-abscisic acid 8'-hydroxylase),紫黄质脱环氧化酶(EC 1.10.99.3,violaxanthin de-epoxidase),15-顺式-八氢番茄红素合成酶(EC 2.5.1.32,15-cis-phytoene synthase),番茄红素环化酶(Cru A),β-胡萝卜素羟化酶(Crt R-b),八氢番茄红素脱饱和酶(Crtl-e)。qRT-PCR分析表明,杜仲愈伤组织中与类胡萝卜素合成相关的九个基因的表达与其转录组高通量测序结果一致,均上调表达。由此推断,强光促进杜仲愈伤组织中类胡萝卜素的积累。本研究可以为获取高产类胡萝卜素资源和杜仲遗传分析提供有价值的资料。
[Abstract]:The high throughput sequencing of eucommia ulmoides callus transcriptional group was analyzed by KEGG pathway. It was found that the callus of Eucommia ulmoides was cultured under intense light (light intensity 12 000 L x ~ (16) h) and dark light for 8 h for 18 days, and dark culture was used as control.Seven enzymes (nine genes) associated with carotenoid synthesis upregulated expression.涓冪�嶉叾鍒嗗埆涓�9-椤哄紡-鐜�姘х被鑳¤悵鍗滅礌鍙屽姞姘ч叄�

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