番茄SlGH3-15基因的功能解析

发布时间：2018-04-04 11:07

本文选题：番茄　切入点：SlGH3-15　出处：《华中农业大学》2016年硕士论文

【摘要】：番茄是一种重要的蔬菜,营养丰富,果实多汁,既可以鲜食又可以加工。市场上具有不同优良性状的番茄新品种,受到了越来越多消费者的青睐。控制番茄重要性状基因的发掘以及功能解析对番茄遗传育种和满足番茄多样化的市场需求有重要意义。现代番茄是经过野生小果的醋栗番茄到樱桃番茄的驯化,以及随后的栽培改良演变而来。本实验室前期依靠第二测序技术对300多份番茄核心种质材料进行了重测序,发现在此300多份番茄材料的基因组上有大量的核苷酸变异,这些变异为番茄由野生小果番茄到大果番茄驯化和改良过程中所涉及到受到选择的位点提供了证据。通过比较分析,课题组在番茄第12染色体的受选择区域上发现了一个SlGH3-15基因,根据其所在染色体的位置,推测其可能与番茄果实的发育相关。本研究通过转基因实验,对SlGH3-15基因的生物学功能进行解析,得到了如下结论：1、在番茄中超量表达SlGH3-15基因,转基因植株的形态发生显著的变化,植株整体矮化,节间距变短,叶片卷曲,侧根变少,并且在植株生长初期植株的侧根的数目就比对照材料少,而且这些变化有显著的差异；2、在番茄中超量表达SlGH3-15基因,转基因植株体内自由态IAA含量比对照材料减少,并且具有显著差异；3、在番茄中干涉SlGH3-15基因表达,转基因植株形态与对照植株没有明显差异,此外转基因植株体内自由态IAA含量与对照也没有显著差异；4.SlGH3-15基因主要在细胞核中和细胞膜上行使功能,并且超量表达的番茄植株叶片细胞层数比对照植株少,细胞形态与对照材料也存在差异；5、外源喷施NAA能够部分恢复超量表达SlGH3-15基因转基因株系的表型。
[Abstract]:Tomato is an important vegetable, rich in nutrition, juicy fruit, can be both fresh and processing.New tomato varieties with different excellent characters are favored by more and more consumers on the market.The discovery of genes controlling the importance of tomato and its function analysis are of great significance to tomato genetics and breeding and to meet the market demand of tomato variety.Modern tomatoes have evolved from wild currant tomatoes to cherry tomatoes, as well as subsequent cultivation improvements.In our laboratory, we resequenced more than 300 tomato core collection materials by the second sequencing technique, and found that there were a lot of nucleotide variations in the genome of more than 300 tomato materials.These variations provide evidence for the selection of sites involved in the domestication and improvement of tomato from wild small fruit tomato to large fruit tomato.Through comparative analysis, we found a SlGH3-15 gene in the selected region of tomato chromosome 12. According to the location of the chromosome, we speculated that it might be related to the development of tomato fruit.In this study, the biological function of SlGH3-15 gene was analyzed by transgenic experiment, and the following conclusions were obtained: 1. Overexpressing SlGH3-15 gene in tomato, the morphology of transgenic plants changed significantly, the whole plant was dwarfed, and the internode spacing became shorter.Leaves curled, lateral roots became less, and the number of lateral roots in the early growth stage of the plant was less than that of the control, and there was significant difference in these changes. The SlGH3-15 gene was overexpressed in tomato.The content of free IAA in transgenic plants was lower than that of control plants, and there was significant difference between transgenic plants and control plants. There was no significant difference in the expression of SlGH3-15 gene between transgenic plants and control plants, and there was no significant difference in morphology between transgenic plants and control plants.In addition, there was no significant difference in free state IAA content between transgenic plants and control plants. 4. SlGH3-15 gene mainly functioned in nucleus and cell membrane, and the number of cell layers in leaves of transgenic plants was less than that of control plants.There was also a difference between the cell morphology and the control material. Exogenous NAA spraying could partially restore the phenotype of transgenic lines expressing SlGH3-15 gene.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S641.2

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