针刺对应激性高血压前期大鼠肾脏基因表达的影响

发布时间：2018-04-05 07:32

本文选题：应激性高血压　切入点：高血压前期　出处：《长春中医药大学学报》2016年06期

【摘要】：目的以应激性高血压前期大鼠为模型,运用基因芯片技术和生物信息学分析技术,研究针刺对其肾脏基因表达的影响。方法将9周龄雄性Wistar大鼠,随机分为空白组、模型组、针刺组。模型组和针刺组运用噪声结合足底电击刺激的造摸方式,造模同时,针刺组针刺曲池穴、太冲穴进行干预,模型组和空白组每天只做与针刺组相同的抓取刺激。记录在造模前1天、第3、5、7、9、11天大鼠的血压变化,11 d后取大鼠左肾。运用Gene Chip Rat Gene 2.0 ST芯片对应激性高血压前期模型大鼠肾组织进行基因表达谱的检测,分别找出空白组与模型组之间及模型组与针刺组之间的差异表达基因。运用GO数据库对各组间差异表达基因进行基因功能富集分析。结果造模开始后,空白组血压基本稳定;第3天开始模型组血压开始上升,与正常组比较有统计学意义(P0.01),并出现行为改变。针刺干预第5天开始,针刺组血压低于模型组(P0.05)。芯片分析显示:与空白组相比,模型组共筛选出的差异表达基因有46个上调,有48个下调;与模型组相比,针刺治疗后差异表达基因有11个上调,有45个下调;基因功能富集分析显示差异基因与生物调节,刺激反应,多细胞生物体过程,代谢过程,免疫系统进程,受体活性,调节酶,转运蛋白活性等功能有关。结论针刺太冲、曲池穴对于应激性高血压前期大鼠具有一定的降压效应,并可能通过下调Cntn4,Spta1,Mir10,Fbxo15基因来调节血压并对其早期肾脏损害有一定的保护作用。
[Abstract]:Objective to study the effect of acupuncture on gene expression in kidney of stress prehypertensive rats by using gene chip technique and bioinformatics analysis technique.Methods 9 week old male Wistar rats were randomly divided into blank group, model group and acupuncture group.Model group and acupuncture group used noise combined with plantar electric stimulation to make model. At the same time, acupuncture group needled Quchi point and Taochong point to intervene. Model group and blank group only did the same grab stimulation as acupuncture group every day.The changes of blood pressure were recorded on the first day before the model and on the 11th day after the 3rd day of the model and the left kidney of the rats were taken out.Gene Chip Rat Gene 2.0ST microarray was used to detect the gene expression profile in kidney tissue of prehypertensive rats. The differentially expressed genes were found between the blank group and the model group and between the model group and the acupuncture group.The gene function enrichment of differentially expressed genes among groups was analyzed by go database.Results the blood pressure of the blank group was basically stable after the model was made, and the blood pressure of the model group began to rise on the 3rd day, which had statistical significance compared with the normal group (P 0.01), and the behavior of the model group was changed.The blood pressure of acupuncture group was lower than that of model group.Chip analysis showed that 46 differentially expressed genes were up-regulated and 48 down-regulated in the model group compared with the blank group, 11 genes were up-regulated and 45 down-regulated after acupuncture compared with the model group.Gene function enrichment analysis showed that differentially expressed genes were related to biological regulation, stimulatory response, multicellular biological processes, metabolic processes, immune system processes, receptor activities, regulatory enzymes, transporter activities, and so on.Conclusion Acupuncture at Taichong and Quchi points has a certain hypotensive effect on prehypertensive rats and may regulate blood pressure and protect early renal damage by down-regulating Cntn4Spta1 Mir10Fbxo15 gene.
【作者单位】：北京中医药大学针灸推拿学院;
【基金】：国家自然科学基金“基于系统生物学的针刺对应激性前期高血压的调控机制与干预靶点的研究”(81373727)
【分类号】：R245

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