粗糙脉孢菌无性产孢过程中增量表达基因的功能分析

发布时间：2018-04-05 23:01

本文选题：粗糙脉孢菌　切入点：无性产孢　出处：《菌物学报》2017年03期

【摘要】：无性产孢是丝状真菌主要的繁殖方式,也是病原真菌传播的基础。为了全面分析丝状真菌无性产孢的调控机制,我们以粗糙脉孢菌为模式菌株,利用RNA seq比较了诱导无性产孢前后的转录组变化。对诱导前后差异表达基因的聚类分析发现,无性产孢诱导主要影响氧化还原过程与代谢过程,与ROS(reactive oxygen species)相关的基因差异表达明显,无性产孢诱导阶段伴随ROS的升高。同时,与产孢相关的基因(包括调控基因和结构基因)出现特异性表达。为揭示其他诱导表达基因在无性产孢中的功能,我们对这些基因缺失突变体的无性产孢表型进行了分析,新发现6个正向影响无性产孢的基因[NCU09792、NCU05159、NCU06112、NCU05079、NCU00461、NCU07521(fwd 2)]。这6个基因在无性产孢过程中增量表达,相应的基因突变体无性产孢量与野生菌株相比有明显降低,说明它们对无性产孢具有正调控作用。以上结果进一步加深了我们对粗糙脉孢菌无性产孢发育及其调控网络的认识。
[Abstract]:Asexual sporulation is the main reproductive mode of filamentous fungi and the basis for the transmission of pathogenic fungi.In order to fully analyze the regulatory mechanism of asexual sporulation of filamentous fungi, we compared the transcriptional changes before and after asexual sporulation induction by RNA seq.Cluster analysis of differentially expressed genes before and after induction showed that asexual sporulation mainly affected the redox process and metabolism, and the differentially expressed genes related to ROS(reactive oxygen specieswere obvious. The asexual sporulation induction was accompanied by the increase of ROS.At the same time, the genes related to sporulation, including regulatory genes and structural genes, were expressed specifically.In order to reveal the function of other inducible expression genes in asexual sporulation, we analyzed the asexual sporulation phenotype of these gene deletion mutants, and found six genes that have positive effect on asexual sporulation [NCU09792NCU05159NCU06112NCU05079NCU00461NCU07521fwd 2].The six genes were expressed incrementally during asexual sporulation, and the asexual sporulation capacity of the corresponding mutant was significantly lower than that of wild strains, which indicated that they had positive regulation on asexual sporulation.The above results further deepen our understanding of asexual sporulation development and regulatory network of C. crassa.
【作者单位】：齐鲁工业大学食品科学与工程学院;中国科学院微生物研究所真菌学国家重点实验室;
【基金】：国家自然科学基金(31370024)~~
【分类号】：Q949.32

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