新疆部分小麦材料籽粒硬度基因等位变异的分子鉴定及其分布

发布时间：2018-04-07 15:10

本文选题：新疆小麦　切入点：Puroindoline基因　出处：《新疆农业科学》2016年06期

【摘要】：【目的】明确小麦籽粒硬度基因Puroindoline基因的等位变异组成与分布,比较新疆当地小麦材料与外引小麦材料硬度基因之间的差别,为改良新疆小麦品种奠定基础。【方法】用Pina-D1b、Pinb-D1b和候补基因非Pinb-D1b等位基因的分子标记,对新疆种植小麦材料的籽粒硬度基因PINA和PINB的分布情况进行分子鉴定。【结果】在鉴定的263份材料中,含Pina-D1b基因的为19.77%,含Pinb-D1b的为29.28%,含互补基因非Pinb-D1b为70.72%;Pina-D1b与Pinb-D1b基因类型在冬春小麦材料中分布不尽相同,Pina-D1b突变基因类型中,春小麦与冬小麦分别为52.68%与1.76%;在Pinb-D1b突变基因类型中,为12.9%与38.23%;Pina-D1b突变基因在新疆当地材料、其他国内材料和国外材料中的频率分别为28.57%、1.89%和33.96%,而Pinb-D1b基因类型的分布,不同来源材料出现的频率差异不明显。【结论】新疆小麦材料中含有突变类型Pina-D1b基因与Pinb-D1b基因的分布。Pina-D1b、Pinb-D1b和候补基因非PinbD1b基因标记引物,可作为小麦籽粒硬度基因鉴定的有效工具,提高小麦品质的选择效率。
[Abstract]:[objective] to determine the allelic variation composition and distribution of Puroindoline gene of wheat grain hardness gene, and to compare the difference of hardness gene between native wheat and imported wheat in Xinjiang.[methods] the molecular markers of Pina-D1b Pinb-D1b and alternate gene non-#en0# alleles were used to improve wheat varieties in Xinjiang.The distribution of grain hardness genes PINA and PINB in Xinjiang cultivated wheat was identified by molecular analysis.The frequencies of Pina-D1b gene mutation in Xinjiang were 12.9% and 38.23% respectively, and the frequencies of other domestic and foreign materials were 28.571.89% and 33.96%, respectively.[conclusion] the distribution of mutant type Pina-D1b gene and Pinb-D1b gene. Pina-D1b Pinb-D1b and alternate gene non-#en2# marker primers were found in Xinjiang wheat.It can be used as an effective tool to identify wheat grain hardness gene and improve the selection efficiency of wheat quality.
【作者单位】：新疆农业科学院粮食作物研究所;
【基金】：新疆维吾尔自治区公益性科研院所基本科研业务费专项(KY2014029) 新疆维吾尔自治区研究与技术开发计划重大专项(201230116-1)~~
【分类号】：S512.1

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