参苓白术散对脾虚湿困型溃疡性结肠炎模型大鼠IL-1β、IL-4及p38MAPK基因蛋白表达的影响

发布时间：2018-04-07 00:14

本文选题：参苓白术散　切入点：溃疡性结肠炎　出处：《中药药理与临床》2017年01期

【摘要】：目的:研究参苓白术散对脾虚湿困型溃疡性结肠炎(UC)模型大鼠结肠黏膜组织内IL-1β、IL-4及p38MAPK基因和蛋白表达的影响。方法:SPF级Wistar大鼠48只,雌雄各半,按随机数字表法分为空白组(n=8)和模型组,采用内外综合因素法复制脾虚湿困型UC大鼠模型,造模成功后将大鼠按随机数字表法分为模型组、参苓白术散组(24g/kg、12g/kg、6g/kg)和柳氮磺胺吡啶组(0.2g/kg),每组8只;其中空白组、模型组大鼠每日按容积10g/kg体重灌服蒸馏水;参苓白术散各组每日分别给予24g/kg、12g/kg、6g/kg剂量的参苓白术散煎剂灌胃;柳氮磺胺吡啶组给予0.2g/kg剂量的柳氮磺胺吡啶药液灌胃,连续治疗3周后,ELISA法检测各组大鼠结肠组织内IL-1β、IL-4含量;q PCR法检测结肠组织内IL-1βmRNA、IL-4 mRNA及p38MAPK mRNA表达;Westernblot法检测结肠组织内p38MAPK蛋白表达。结果:与空白组比较,模型组大鼠一般生存状况较差,结肠组织内IL-1β含量及IL-1βmRNA表达水平显著升高,IL-4含量及IL-4 mRNA表达水平明显降低(P0.05),p38MAPK基因和蛋白表达水平明显升高;与模型组比较,参苓白术散组大鼠一般生存状况明显改善,结肠组织内IL-1β含量及IL-1βmRNA表达水平明显降低,IL-4含量及IL-4mRNA表达水平明显升高,p38MAPK基因和蛋白表达水平明显降低,以参苓白术散治疗组(24g/kg)和柳氮磺胺吡啶组(0.2g/kg)治疗作用明显;参苓白术散治疗组(24g/kg)和柳氮磺胺吡啶组(0.2g/kg)相比较,二者之间差异无统计学意义。结论:参苓白术散水煎液(24g/kg、12g/kg)具有改善脾虚湿困型UC大鼠一般生存状况,并通过上调结肠组织IL-4蛋白含量及其mRNA表达水平,下调IL-1β和p38MAPK基因蛋白表达水平而发挥保护结肠黏膜的作用。
[Abstract]:Aim: to study the effect of Shenling Baizhu Powder on the expression of IL-1 尾 -IL-4, p38MAPK gene and protein in colonic mucosa of rats with spleen deficiency and damp-trapped ulcerative colitis.Methods Forty-eight Wistar rats, half male and half female, were randomly divided into blank group (n = 8) and model group (n = 8). The model of UC rats with spleen deficiency and dampness was induced by internal and external comprehensive factors. After the establishment of the model, the rats were divided into two groups according to the random digital table method.The salicylate sulfamyridine group was given 0.2g/kg dose of sulfamyridine solution intragastrically.After 3 weeks of continuous treatment, the expression of IL-1 尾 -mRNA-IL-4 mRNA and p38MAPK mRNA in colonic tissue were detected by Elisa method and the expression of IL-1 尾 -mRNA-IL-4 mRNA and p38MAPK mRNA in colon tissue of rats in each group. The expression of p38MAPK protein in colon tissue was detected by Western blot.Results: compared with the blank group, the general living condition of the model group was poor. The expression of IL-1 尾 and IL-1 尾 mRNA in colonic tissue was significantly increased. The content of IL-4 and the expression level of IL-4 mRNA were significantly decreased. The expression of p38 MAPK gene and protein was significantly increased in the model group.Compared with the model group, the general living condition of the rats in the Shenling Atractylodes Powder group was obviously improved, and the IL-1 尾 content and the IL-1 尾 mRNA expression level in the colonic tissue were significantly decreased. The level of IL-4 and IL-4mRNA expression was significantly increased and the expression level of p38 MAPK gene and protein was obviously decreased.There was no significant difference between Shenling Baizhu Powder treatment group (24g / kg) and salbutamulfonridine group (0.2g / kg) and Shenling Baizhu powder treatment group (24g / kg) and salicylosulfamyridine group (0.2g / kg).Conclusion: Shenling Atractylodes Powder decoction can improve the living condition of UC rats with spleen deficiency and dampness, and up-regulate the content of IL-4 protein and the expression of mRNA in colonic tissue.The expression of IL-1 尾 and p38MAPK genes was down-regulated to protect colon mucosa.
【作者单位】：甘肃中医药大学;甘肃省中药药理与毒理学重点实验室;甘肃省人民医院;
【基金】：国家自然科学基金项目(NO.81360518) 甘肃省科技厅自然科学基金B类项目(NO.148RJZA082)
【分类号】：R285.5

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