C3AR1基因对K562细胞增殖及侵袭影响的机制研究

发布时间：2018-04-07 17:59

本文选题：白血病　切入点：髓样　出处：《中国全科医学》2017年S1期

【摘要】：目的探讨C3AR1基因对慢性髓性白血病细胞株K562细胞增殖及侵袭的影响和作用机制。方法构建HL-60-C3AR1过表达的K562细胞株并使其稳转表达,通过反转录聚合酶链式反应(RT-PCR)和Western blotting检测C3AR1的mRNA和蛋白表达水平,采用CCK8法检测转染后细胞的增殖,通过trans well实验评估其侵袭性,通过PCR芯片表达对比分析其可能的机制。结果 K562-C3AR1的C3AR1 mRNA表达和C3AR1蛋白表达量均显著高于K562和K562-Scramble组(P0.05),过表达C3AR1的细胞增殖性和细胞侵袭性显著高于K562和K562-Scramble组(P0.05),PCR芯片检测显示共有7个基因表达增高和4个基因表达下调可能参与K562-C3AR1相对K562发生增殖及侵袭的相关基因。结论本研究表明了C3AR1基因对K562细胞具有重要的调控作用,过表达C3AR1基因能促进K562细胞增殖及侵袭,其具体机制可能与DBH和EPS8等基因有关。
[Abstract]:Objective to investigate the effect and mechanism of C3AR1 gene on proliferation and invasion of chronic myeloid leukemia cell line K562.Methods K562 cell line with overexpression of HL-60-C3AR1 was constructed and stably expressed. The mRNA and protein expression levels of C3AR1 were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and the proliferation of transfected K562 cells was detected by CCK8 assay.The invasiveness was evaluated by trans well experiment, and the possible mechanism was analyzed by PCR chip expression.Results the expression of C3AR1 mRNA and C3AR1 protein in K562-C3AR1 were significantly higher than those in K562 and K562-Scramble groups, and the proliferation and invasiveness of over-expressed C3AR1 were significantly higher than those in K562 and K562-Scramble groups.The down-regulation of gene expression may be involved in the proliferation and invasion of K562-C3AR1 relative to K562.Conclusion this study suggests that C3AR1 gene plays an important role in the regulation of K562 cells. Overexpression of C3AR1 gene can promote the proliferation and invasion of K562 cells, and its specific mechanism may be related to DBH and EPS8 genes.
【作者单位】：遵义医学院附属医院;
【分类号】：R733.72

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