鱼腥草HMGR基因cDNA克

发布时间：2018-04-07 18:05

本文选题：鱼腥草　切入点：-羟基--甲基戊二酰辅酶A还原酶　出处：《中草药》2017年18期

【摘要】：目的克隆鱼腥草3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)基因并分析其差异表达。方法采用实时荧光定量PCR(RT-PCR)方法获得HMGR基因cDNA序列并对HMGR蛋白进行理化性质、蛋白二级结构及三维结构预测分析,并预测该蛋白功能;利用RT-PCR方法检测HMGR基因在鱼腥草的地下茎、地上茎、叶、花中的表达情况。结果克隆获得的HMGR基因cDNA全长为1 626 bp,编码541个氨基酸。生物信息学预测HMGR蛋白含2个跨膜区,不含信号肽。HMGR基因主要在鱼腥草的花中表达,其他器官中表达相对较低,地下茎中表达量最低。结论首次从鱼腥草中克隆了HMGR基因,为进一步阐明该基因在鱼腥草萜类化合物代谢途径中的作用奠定基础。
[Abstract]:Objective to clone and analyze the differentially expressed HMGRs gene of Houttuynia cordata (Houttuynia cordata) 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR).Methods the cDNA sequence of HMGR gene was obtained by real-time fluorescence quantitative PCR, and the physicochemical properties, secondary structure and three dimensional structure of HMGR protein were predicted, and the function of the protein was predicted. RT-PCR method was used to detect HMGR gene in the rhizome of Houttuynia cordata.The expression of stems, leaves, and flowers on the ground.Results the total length of HMGR gene cDNA was 1 626 BP, encoding 541 amino acids.Bioinformatics predicted that HMGR protein contained two transmembrane regions, and no signal peptide. HMGR gene was mainly expressed in the flowers of Houttuynia cordata. The expression of HMGR was relatively low in other organs and the lowest in the rhizome.Conclusion the HMGR gene was cloned from Houttuynia cordata for the first time, which laid a foundation for further elucidating the role of the gene in the metabolic pathway of terpenoids in Houttuynia cordata.
【作者单位】：怀化学院生命科学系民族药用植物资源研究与利用湖南省重点实验室湘西药用植物与民族植物学湖南省高校重点实验室;
【基金】：国家自然科学基金项目(30870230) 湖南省科技计划项目(2013FJ6090,2014FJ4207) 湖南省高校创新平台开放基金项目(15K100) 湖南省生物类专业大学生创新训练中心资助植物学湖南省高校“十二五”重点建设学科资助(ZWX2016-07)
【分类号】：Q943.2;S567.239

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