鸡源多杀性巴氏杆菌的分离鉴定及其毒力基因的检测

发布时间：2018-04-08 15:05

本文选题：多杀性巴氏杆菌　切入点：分离　出处：《中国兽医科学》2017年07期

【摘要】：取病死鸡肝接种于血清琼脂平板,挑取符合巴氏杆菌特征的单菌落进行纯化培养和染色镜检;对纯培养菌提取核酸,进行16S r RNA、kmt1和cap A基因的PCR鉴定,7个管家基因的PCR扩增和多位点序列分型,以及12个毒力基因的PCR检测;并进行分离菌的动物回归试验。结果显示,分离的2株病原菌(HN-1和CZ-6)均为两极浓染的革兰阴性短杆菌,16S r RNA和kmt1基因序列与多杀性巴氏杆菌的同源性均高于99%,荚膜血清型鉴定为A型,基因型为ST129型,除tox A、ptf A、nan H之外的9个毒力基因均被检出,动物回归试验表明分离菌对鸡的致病性较强。结果表明,这2株鸡源多杀性巴氏杆菌的分离鉴定为我国禽源菌株的遗传特性研究提供了一定参考。
[Abstract]:The dead chicken liver was collected and inoculated on the Agar plate of serum, and the single colony with the characteristic of Pasteurella was selected for purification and staining, and nucleic acid was extracted from the pure culture bacteria.The 16s r RNA-kmt1 and cap A genes were identified by PCR, 7 housekeeping genes were amplified by PCR and multilocus sequence typing, and 12 virulence genes were detected by PCR, and the animal regression test of isolated bacteria was carried out.The results showed that the sequences of 16s r RNA and kmt1 genes of Gram-negative short bacilli (HN-1 and CZ-6) were higher than those of Pasteurella multocida. The capsular serotypes were identified as type A and genotypes were ST129.All the 9 virulence genes except tox PTF Agnan H were detected. The animal regression test showed that the isolated bacteria had strong pathogenicity to chicken.The results showed that the isolation and identification of these two strains of Pasteurella multocida from chickens provided some references for the study of genetic characteristics of avian strains in China.
【作者单位】：安徽科技学院动物科学学院;河南省驻马店市畜牧局;
【基金】：安徽省教育厅自然科学研究重点项目(KJ2016A822) 安徽科技学院自然科学研究项目(ZRC2016497)
【分类号】：S852.612

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