中国六省水痘-带状疱疹病毒糖蛋白基因特征分析

发布时间：2018-04-09 07:38

本文选题：水痘-带状疱疹病毒　切入点：糖蛋白　出处：《病毒学报》2017年02期

【摘要】：为研究中国水痘-带状疱疹病毒(Varicella Zoster Virus,VZV)gH(Glycoprotein H)、gK(Glycoprotein K)、gI(Glycoprotein I)糖蛋白基因特征,选取2007~2015年在中国六省收集的VZV疑似患者的疱疹液或咽拭子标本,经聚合酶链反应(Polymerase chain reaction,PCR)方法进行阳性鉴定及3个糖蛋白的全基因片段扩增,所得序列与GenBank中已公布的疫苗株及野毒株序列进行比对分析。结果筛选出12份阳性VZV标本,gH、gK、gI与水痘疫苗株核苷酸和氨基酸同源性相比分别为99.8%~100%、99.8%~100%,99.8%~100%、99.6%~100%,99.9%~100%、99.7%~100%;与疫苗株相比在gH、gK、gI糖蛋白基因位点上12份阳性标本分别至少有一个核苷酸差异;与GeneBank中已报告的野毒株相比核苷酸和氨基酸同源性分别为99.6%~100%、99.3%~100%,99.7%~100%、99.3%~100%,99.7%~100%、99.7%~100%。本研究通过分析中国六省VZV糖蛋白gH、gK、gI的基因特征,发现这三个糖蛋白高度保守,抗原性稳定,为了解中国水痘带状疱疹病毒野毒株糖蛋白本底资料,丰富基因数据,从分子水平上为疫苗效果的评估、基因组学的研究提供基础数据。但中国地域辽阔,人口众多,还需每年连续监测并进一步扩大VZV病毒监测的地域。
[Abstract]:For the study of Chinese varicella zoster virus (Varicella Zoster, Virus, VZV) gH (Glycoprotein H), gK (Glycoprotein K), gI (Glycoprotein I) glycoprotein gene characteristics, selection of 2007~2015 collected in Chinese province six suspected VZV patients with herpes or throat swab specimens, by polymerase chain reaction (Polymerase chain reaction, PCR) amplified the whole gene fragment for positive identification and 3 glycoproteins, vaccine strains and wild strains of published sequences and GenBank sequences obtained were compared and analyzed. The result showed that 12 positive specimens of VZV, gH, gK, gI compared with the varicella vaccine strain nucleotide and amino acid homology were 99.8%~100% 99.8%~100%, 99.8%~100%, 99.6%~100%, 99.9%~100%, and 99.7%~100%; compared with the vaccine strain in gH, gK, gI locus glycoprotein gene of 12 positive specimens were at least one nucleotide difference; wild strain and GeneBank have been reported Nucleotide and amino acid homology were 99.6%~100%, compared to 99.3%~100%, 99.7%~100%, 99.3%~100%, 99.7%~100%, 99.7%~100%. through the analysis of China six VZV glycoprotein gH, gK, gI genotype, and found that the three highly conserved glycoprotein, antigenic stability, in order to understand the China varicella zoster virus glycoprotein gene rich background information, the data, evaluation of vaccine effectiveness at the molecular level, and provide basic data for genomic research. But Chinese vast territory, large population, still need to further expand the VZV year continuous monitoring and virus monitoring areas.

【作者单位】：大连医科大学;中国疾病预防控制中心病毒病预防控制所;上海市疾病预防控制中心;
【基金】：国家自然科学基金(项目号:81302477),题目:水痘-带状疱疹病毒基因型在我国的分布及糖蛋白基因特征研究国家重点研发计划课题(项目号:2016YFC1200905),题目:重要新发突发病原体应急处置方案与规范研究
【分类号】：R373.1

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