蒺藜苜蓿中1个聚半乳糖醛酸酶基因突变体的筛选及共生固氮表型的初步鉴定

发布时间：2018-04-09 08:09

本文选题：蒺藜苜蓿　切入点：聚半乳糖醛酸酶基因　出处：《华中农业大学学报》2017年04期

【摘要】：以模式豆科植物蒺藜苜蓿中1个聚半乳糖醛酸酶(polygalacturonase,PG)基因为对象,对其进行生物信息学分析,结果显示:该基因全长4 150bp,其编码区1 278bp,编码425个氨基酸;其编码蛋白包括2个与细胞壁主要成分果胶降解有关的保守结构域PL-6superfamily和Glyco_hydro_28;该酶蛋白与鹰嘴豆、赤豆等豆科植物中的PG蛋白同源关系更近。苜蓿基因表达谱数据库分析和RT-PCR检测结果表明,MtPG在根瘤中呈显著增强表达,在非共生组织如根、叶中表达量很低。此外,基于该基因3个Tnt1转座子插入的目标候选突变体(NF0999、NF5561和NF4746)插入位点分析,得到1个纯合突变体材料NF4746,共生表型观察表明,MtPG基因在豆科植物共生固氮的早期侵染过程中发挥重要作用。
[Abstract]:A polygalacturonase polygalacturonase (PGN) gene from alfalfa of Tribulus terrestris was studied by bioinformatics. The results showed that the whole length of the gene was 4 150 BP, and its coding region was 1 278 BP, encoding 425 amino acids.The encoded proteins include two conserved domains, PL-6superfamily and Glycostace 28, which are related to the degradation of pectin, which is the main component of cell wall. The protease protein is more closely related to PG protein in legumes such as chickpea, red bean and so on.The analysis of alfalfa gene expression profile database and RT-PCR analysis showed that the expression of MtPG in root nodules was significantly enhanced, but in non-symbiotic tissues such as roots and leaves, the expression level was very low.In addition, the insertion sites of the target candidate mutants, NF0999NNF5561 and NF4746, were analyzed based on the insertion of three Tnt1 transposons of the gene.A homozygous mutant NF4746 was obtained. The symbiotic phenotypic observation showed that MtPG gene played an important role in the early infection of symbiotic nitrogen fixation in legumes.
【作者单位】：农业微生物学国家重点实验室/华中农业大学生命科学技术学院;
【基金】：国家重点研发计划项目(2016YFD0100702) 国家自然科学基金项目(31670243;31371549)
【分类号】：Q943.2

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