牛乳头状瘤病毒2型贵州株L2基因克隆及生物信息学分析

发布时间：2018-04-09 23:04

本文选题：牛乳头状瘤病毒型　切入点：贵州株　出处：《中国兽药杂志》2017年08期

【摘要】：为分析牛乳头状瘤病毒2型贵州株(BPV2-GZ01株)L2基因的分子特征,预测编码蛋白的生物学功能,对BPV2-GZ01株L2基因进行PCR扩增、克隆及序列测定,应用生物信息学相关软件及方法,对BPV2-GZ01株L2基因进行序列分析并对其编码蛋白进行二级结构、三级结构、B细胞表位、保守结构域、跨膜结构域和信号肽预测。结果显示,L2基因PCR扩增产物大小为1404 bp,编码467个氨基酸;与参考株BPV2株、BPV2-SW01株、BPV2-AKS01株、BPV13株、BPV1株相应序列核苷酸同源性分别为99.0%、99.7%、99.3%、83.9%和75.1%,氨基酸的同源性分别为98.9%、99.6%、99.4%、89.5%和82.7%;系统进化显示,BPV2-GZ01株L2基因与BPV2-SW01株亲缘关系最近;二级结构以无规则卷曲、β-折叠和α-螺旋区域所占比例较大,预测此蛋白可能存在B细胞优势抗原表位,无跨膜区域,无信号肽区域。本研究结果将为贵州省BPV核酸疫苗研究提供理论依据。
[Abstract]:In order to analyze the molecular characteristics of BPV2-GZ01 strain of bovine papillomavirus type 2 (BPV2-GZ01) and predict the biological function of encoding protein, the L2 gene of BPV2-GZ01 strain was amplified by PCR, cloned and sequenced, and the relevant software and methods of bioinformatics were used.The L2 gene of BPV2-GZ01 strain was sequenced and its encoding protein was predicted by secondary structure, tertiary structure, B cell epitope, conserved domain, transmembrane domain and signal peptide.The results showed that the PCR amplification product of L2 gene was 1404 BP, encoding 467 amino acids.The secondary structure was characterized by irregular curl, 尾 -folding and 伪 -helix. It was predicted that the protein might have B cell epitopes, no transmembrane region and no signal peptide region.The results of this study will provide theoretical basis for the study of BPV nucleic acid vaccine in Guizhou province.
【作者单位】：贵州大学动物科学学院;贵州轻工职业技术学院;贵州省六盘水市动物卫生监督所;贵州省德江县动物疫病预防控制中心;
【基金】：贵州省黔南州社会发展科技重大计划项目[黔南社发科(20140325)] 贵州省农业委员会科技专项(黔农财[2015]149号)
【分类号】：S852.65

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