流体剪切力对人脂肪基质细胞成骨相关基因表达影响的研究

发布时间：2018-04-10 04:31

本文选题：流体剪切力　切入点：脂肪基质细胞　出处：《国际口腔医学杂志》2017年02期

【摘要】：目的探讨流体剪切力(FSS)对人脂肪基质细胞(ADSC)成骨相关基因骨钙素(OCN)、转录因子Osterix(Osx)及核心结合因子(Cbf)a1 / Runt相关转录因子(Runx)2表达的影响。方法将脂肪抽吸术获取的人体脂肪进行常规培养(A1、A2组)和诱导培养(A3、A4组),然后分别对其加载强度为0.3 Pa的FSS(A1、A3组),未加FSS的为对照组(A2、A4组),通过逆转录-聚合酶链反应检测骨钙素、转录因子Osx及Cbfa1/Runx2基因表达的情况。结果 A3、A4组在FSS加载后,其成骨相关基因的表达增强;而A1、A2组未见成骨特异性基因条带。这表明在常规培养条件下,无论是否有FSS的刺激,均无成骨特异性基因表达。结论脂肪基质细胞在诱导培养条件下,FSS可促使其向成骨细胞分化,可作为骨组织工程的种子细胞。
[Abstract]:Objective to investigate the effects of fluid shear stress (FSS) on the expression of osteoblast-associated gene osteocalcin (OCN), transcriptional factor Osterix Osx (Osx) and the core binding factor CbfA-1 / Runt related transcription factor (Runxt2) in human adipose stromal cells (ADSCs).Methods Human fat obtained by liposuction was cultured in routine culture group A _ (1) A _ (2) and induced culture group A _ (3) A _ (4), and then loaded with FSSA _ (1) A _ (1) A _ (3) group with a strength of 0.3 Pa and control group (group A _ (2) A _ (4) with no FSS added) by reverse transcription-polymerase chain reaction (RT-PCR).Reaction detection of osteocalcin,Expression of Osx and Cbfa1/Runx2 genes.Results the expression of osteoblast-associated genes was increased in group A _ 3 and A _ (3) A _ (4) after FSS loading, while no band of osteoblast-specific genes was found in group A _ (1) A _ (2).This indicated that there was no osteoblast-specific gene expression under conventional culture conditions, whether or not stimulated by FSS.Conclusion FSS can induce adipose stromal cells to differentiate into osteoblasts and can be used as seed cells for bone tissue engineering.
【作者单位】：深圳市北京大学深圳医院口腔科;
【基金】：深圳市知识创新计划基础研究项目(20130320180202)~~
【分类号】：R781

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