大麦赤霉病抗性不同突变体的创制及其相关基因表达

发布时间：2018-04-10 09:55

本文选题：大麦　切入点：赤霉病　出处：《核农学报》2017年03期

【摘要】：为了快速创制大麦赤霉病抗性存在差异的加倍单倍体(DH)株系,采用单花滴注法和喷雾法对诱变结合小孢子培养的379份大麦DH株系和原始品种花30的赤霉病抗扩展性和抗侵染性进行评价,同时对获得的抗性突变体材料和花30进行赤霉菌诱导下的大麦病程相关基因非表达子(NPR1、NPR2、NPR3)、茉莉酸介导的植物防御的调控因子(JAV1)以及乙烯响应因子(ERF1)的基因表达分析。结果表明,通过连续2年大田人工接种鉴定,从中获得了5份抗扩展性和抗侵染性均优于花30的候选株系,9份抗扩展性和抗侵染性均劣于花30的候选株系;赤霉病抗性突变体A1-190与原始品种花30在上述基因表达上存在明显差异,推测这些基因的表达与其抗病性的变化相关。研究结果表明诱变结合小孢子培养可以创制的DH株系在大麦赤霉病抗性存在差异。本研究结果为大麦抗赤霉病基因定位克隆及分子育种奠定了材料基础。
[Abstract]:In order to quickly create double haploid DH lines with different resistance to barley scab,Single flower drip method and spray method were used to evaluate the expansibility and resistance to scab of 379 barley DH strains and original cultivar Flower 30 cultured by mutagenesis and microspore culture.At the same time, the resistant mutant and flower 30 obtained were analyzed for gene expression of the non-expressed gene NPR1, NPR2, NPR2, JAV1, and ethylene response factor, ERF1, induced by gibberellis in barley (NPR1, NPR2, NPR3), jasmonic acid mediated plant defense regulatory factor (JAV1) and ethylene responsive factor (ERF1).The results showed that, after 2 years of artificial inoculation in the field, 5 strains with expansibility and resistance to infection were better than those of Huayu 30, and 9 of them were inferior to those of Huayu 30.There were significant differences in the expression of the above genes between the resistant mutant A1-190 and the original cultivar Flower 30. It was speculated that the expression of these genes was related to the change of resistance to scab.The results showed that the resistance of DH strain which could be created by mutagenesis combined with microspore culture was different in barley scab.The results of this study laid a material foundation for location cloning and molecular breeding of scab resistant genes in barley.
【作者单位】：上海市农业科学院生物技术研究所/上海市农业遗传育种重点实验室;苏州农业职业技术学院;
【基金】：上海市农口青年系统青年人才成长计划(沪农青字(2015)第1-29) 上海市科委基础重点项目(14JC1405300) 上海市种业发展项目[沪农科种字(2016)第1-1号] 大麦青稞产业技术体系(CARS-05)
【分类号】：S435.123

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