拟南芥QUA1基因在光信号途径中的表达与功能分析

发布时间：2018-04-11 16:05

本文选题：拟南芥 + QUA　；参考：《遗传》2016年05期

【摘要】：光信号在植物生长发育过程中具有非常重要的作用。不同的光信号通过调节植物下游基因的表达,进而影响细胞分化、结构和功能的改变,以及组织和器官的形成,参与植物光形态建成。QUA1(QUASIMODO1)是拟南芥糖基转移酶家族中的一个成员,参与植物细胞壁中果胶的合成。本文以拟南芥qua1-1/cry1以及qua1-1/phy B双突变体为材料,对QUA1基因在光信号途径中的功能进行了分析。结果显示,qua1-1突变体在暗、蓝光、红光以及远红外光培养条件下下胚轴的伸长均受到抑制,QUA1基因的表达同样受到光信号的调节,而且突变体中多种光信号调节基因的表达也受到了影响。通过对qua1-1突变体下胚轴的观察发现,突变体下胚轴表皮细胞长度明显变短。与cry1以及phy B突变体相比,qua1-1/cry1和qua1-1/phy B双突变体下胚轴长度明显变短,而且双突变体中光信号调节基因的表达也有明显变化,表明QUA1可能参与了CRY1以及PHYB介导的蓝光及红光信号传导。以上结果表明QUA1影响了下胚轴细胞的伸长以及光信号调节基因的表达,并参与调控多种光信号传导途径。
[Abstract]:The optical signal plays a very important role in plant growth and development. The optical signal by regulating the expression of downstream genes of different plants, thereby affecting cell differentiation, the change of structure and function as well as the formation of tissues and organs involved in plant photomorphogenesis,.QUA1 (QUASIMODO1) is a member of the Arabidopsis glycosyltransferase enzyme family the synthesis in plant cell wall pectin. In this paper, the qua1-1/phy B double mutant qua1-1/cry1 of Arabidopsis thaliana as material, the function of QUA1 gene in light signaling pathways were analyzed. The results showed that the qua1-1 mutant in the dark, blue light, red light and far infrared light training under the condition of hypocotyl elongation was inhibited, the expression of QUA1 the same gene is regulated by light signal, and a variety of optical signals in the mutant regulate gene expression were also affected. The qua1-1 mutant hypocotyls view Police found that the mutant hypocotyl epidermal cells was significantly shorter. Compared with cry1 and PHY B qua1-1/cry1 and qua1-1/phy B double mutant, mutant hypocotyl length becomes shorter, and the double mutant signal regulate gene expression also changed significantly, suggesting that QUA1 may be involved in CRY1 and PHYB mediated blue light and red light signal conduction. These results showed that QUA1 affected the hypocotyl cell elongation and the optical signal to regulate gene expression, and involved in the regulation of a variety of light signal transduction pathway.

【作者单位】：南阳师范学院农业工程学院;
【基金】：国家自然科学基金项目(编号:31400264)资助~~
【分类号】：Q943.2

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