苹果矮化砧木GA20氧化酶基因的克隆及其表达分析

发布时间：2018-04-12 00:34

本文选题：苹果矮化砧木 + GA氧化酶　；参考：《华北农学报》2017年01期

【摘要】：为了研究GA20氧化酶基因在苹果矮化砧木中的分子特征和表达特征,利用RT-PCR方法从苹果矮化砧木2号和36号c DNA中克隆了GA20氧化酶基因(GA20ox1),并对该基因及其编码氨基酸序列以及在不同时期砧木及嫁接品种中的表达分别进行了分析。结果表明,GA20ox1基因c DNA编码序列长1 179 bp,推导编码393个氨基酸(包括终止密码子),预测蛋白相对分子质量44.3 k Da,理论等电点5.89,编码的蛋白质包含GA20ox1基因家族所具有的特征保守结构域,系统进化分析表明该蛋白序列与苹果SH40的同源性达到96.9%。实时荧光定量PCR分析显示:36号砧木在6月份GA20ox1基因表达强度显著低于八棱海棠对照,而7-8月份的均显著高于对照,其上嫁接的品种7月份表达强度显著低于对照。在7-8月份2号砧木及嫁接品种GA20ox1基因的表达强度均显著低于对照。
[Abstract]:In order to study the expression characteristics of GA20 oxidase gene in Apple Dwarf Rootstock and molecular characteristics, using the method of RT-PCR from Apple Rootstock No. 2 and No. 36 C DNA clone GA20 oxidase gene (GA20ox1), and the gene and its encoding amino acid sequence and expression in different periods of rootstock and grafted varieties in were analyzed respectively.. the results showed that the GA20ox1 gene C encoding DNA sequence length of 1179 BP deduced encoding 393 amino acids (including termination codon), prediction of protein with a relative molecular mass of 44.3 K Da, the theoretical isoelectric point of 5.89, the characteristics of conserved domain encoding proteins containing the GA20ox1 gene family with the phylogenetic analysis indicated that the protein sequence and apple SH40 is homologous to the real-time fluorescence quantitative PCR 96.9%. analysis showed that the 36 rootstocks in June GA20ox1 gene expression intensity was significantly lower than the eight shoots while 7-8 months were control. The expression level of the grafted varieties in July was significantly lower than that of the control. In 7-8 months, the expression intensity of GA20ox1 gene in 2 rootstocks and grafted varieties was significantly lower than that in the control.

【作者单位】：河北农业大学园艺学院;
【基金】：河北省科技厅科技支撑项目(14226307D-5)
【分类号】：S661.1

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