新疆维吾尔族自治区汉族与维吾尔族MMP-13基因多态性与骨性关节炎的关联性

发布时间：2018-04-12 10:57

本文选题：MMP- + 骨性关节炎　；参考：《广东医学》2017年21期

【摘要】：目的采用病例-对照研究,分析新疆地区汉族与维吾尔族骨性关节炎(OA)患者与非骨性关节炎(NOA)人群的基质金属蛋白酶-13(MMP-13)基因启动子区转录起始点上游77bp处A/G多态等位基因和基因型频率分布的差异,探讨此位点基因多态性与OA遗传易感性的关系。方法采用聚合酶链反应(PCR)限制性片段长度多态性技术,对维吾尔族年龄(57.54±0.35)岁、体质指数(BMI)(24.27±0.11)kg/m~2的绝经后女性334例和汉族年龄(54.74±0.30)岁、BMI(24.12±0.09)kg/m~2的绝经后女性468例进行MMP-13A-77G基因检测。结果汉族及维吾尔族MMP-13A-77G基因型分布符合Hardy-Weinberg平衡(P0.05),MMP-13 SNP的等位基因型频率在汉族与维吾尔族组总体分布差异有统计学意义(χ~2=31.185,P0.001);维吾尔族人群中MMP-13A-77G 3种基因型频率在OA组及NOA组之间差异有统计学意义(P0.001),且与AA基因型相比AG、GG基因型可以显著降低OA的发病风险(χ~2=0.442、0.428;P0.001),经年龄、BMI调整后(95%CI=0.066~0.371和0.090~0.481;OR=0.156和0.208);汉族人群中MMP-13A-77G AA、GG基因型频率在OA组及NOA组之间差异有统计学意义(P0.001),AG基因型在OA和NOA组之间差异无统计学意义(P=0.691),且与AA基因型相比GG基因型可以显著增加OA的发病风险(χ~2=0.447;P0.001),经年龄、BMI调整后(95%CI=2.640~15.213;OR=6.338)。结论年龄与BMI是OA的危险因素,随着年龄及BMI的增加,OA的患病率也在增加。维吾尔族MMP-13 SNP基因多态性分布与汉族相比差异有统计学意义(P0.001)。
[Abstract]:Objective to adopt a case-control study,To analyze the difference of the distribution of A / G polymorphism allele and genotype in the 77bp of the promoter region of matrix metalloproteinase-13 (MMP-13) gene between Han and Uygur patients with osteoarthritis (OAA) and non-osteoarthritis (NOAA) in Xinjiang.To investigate the relationship between genetic polymorphism of this locus and genetic susceptibility to OA.Results the distribution of MMP-13A-77G genotype in Han nationality and Uygur nationality was in accordance with the allele frequency of Hardy-Weinberg balanced P0.05MMP 13 SNP in Han nationality and Uygur nationality. There was significant difference between Han nationality and Uygur nationality in the total distribution of MMP-13A-77G genotype (蠂 2 + 31.185%, P 0.001), and the frequencies of three MMP-13A-77G genotypes in Uygur population in OA group were significantly higher than those in control group (P < 0.05).Conclusion Age and BMI are risk factors of OA, and the prevalence of OA increases with the increase of age and BMI.The polymorphism distribution of MMP-13 SNP gene in Uygur nationality was significantly different from that in Han nationality (P 0.001).
【作者单位】：石河子大学医学院;石河子大学医学院第一附属医院关节外科;
【基金】：国家自然科学基金资助项目(编号:81160225) 兵团医药卫生专项课题(编号:2013BA020) 兵团国际交流与合作专项基金(编号:2012BC002) 兵团骨科创新团队专项(编号:2014BC003)
【分类号】：R684.3

【相似文献】