基于Omp1基因的泌尿生殖道感染沙眼衣原体多样性及分布特征分析

发布时间：2018-04-12 13:04

本文选题：沙眼衣原体 + 泌尿生殖道　；参考：《中国皮肤性病学杂志》2017年04期

【摘要】：目的基于主要外膜蛋白基因(Omp1)研究泌尿生殖道感染患者沙眼衣原体多态性及分布特征,为该疾病的诊治及疫苗研究奠定基础。方法对泌尿生殖道感染临床标本常规处理后,使用沙眼衣原体核酸检测试剂盒筛选阳性标本,然后以巢式PCR扩增Omp1基因,选择4个限制性内切酶进行RFLP分析确定其基因型,选择代表菌株测定Omp1的DNA序列并与参比菌株比较确定其血清型,最后将基因型及血清型与患者的性别、科室及年龄进行对比分析,研究其分布特征。结果 2013-2014年2年间共筛选得到178份阳性标本,61份标本扩增获得Omp1基因,Omp1-PFLP分析将61份标本分为6个基因型,占比分别为24.59%,24.59%,4.92%,9.84%,3.28%和32.79%,血清型分析表明61份标本属于F,H,J,G,Da,D和E 7种血清型,E型及F型占比最多。结论本地区引起泌尿生殖道感染的沙眼衣原体主要属于F,H,J,G,Da,D和E 7种血清型,E型及F型占比最多,基因型及血清型与患者性别具有相关性,与科室、年龄无相关性。
[Abstract]:Objective to study the polymorphism and distribution of chlamydia trachomatis in patients with genitourinary tract infection based on the main outer membrane protein gene (Omp1) so as to lay a foundation for the diagnosis and treatment of the disease and the study of vaccine.The representative strains were selected to determine the DNA sequence of Omp1 and compared with the reference strains to determine the serotype. Finally, the genotypes and serotypes were compared with the patients' sex, department and age, and their distribution characteristics were studied.Results from 2013 to 2014, a total of 178 positive specimens were selected and 61 samples were amplified to obtain Omp1 gene Omp1-PFLP analysis. 61 samples were divided into 6 genotypes.The percentage of serum type was 3.28% and 32.79%, respectively. The analysis of serum type showed that 61 samples belonged to the seven serotypes (E type E and F type).Conclusion Chlamydia trachomatis caused by genitourinary tract infection mainly belongs to the seven serotypes of FHG, E, E, E, E, E and E, and genotype and serotype are correlated with sex of patients, but not related to department, age.
【作者单位】：河北北方学院病原生物学研究所;保定市第一中心医院检验科;中国人民解放军第二五二医院检验科;
【基金】：河北北方学院创新人才项目(CXRC1317);河北北方学院青年基金(Q2013030)
【分类号】：R440

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