路易斯安那鸢尾LiNramp2基因克隆与定量表达分析

发布时间：2018-04-12 13:10

本文选题：路易斯安那鸢尾 + Pb胁迫　；参考：《热带作物学报》2016年01期

【摘要】：利用RACE技术克隆了路易斯安那鸢尾LiNramp2基因全长cDNA序列,其大小为1 786 bp,预测编码519个氨基酸,蛋白质具有11次跨膜结构域,与其他Nramp蛋白同源性达80%以上,说明LiNramp2同其他同源基因保守性很高。定量表达结果显示,LiNramp2在路易斯安那鸢尾叶片和雄蕊中表达量较高,在雌蕊中表达量最低,根和茎表达量差别不大。随着铅处理时间增加,LiNramp2基因在根系表现缓慢下降,在叶片中表现先升高后下降的趋势,但差异不显著。
[Abstract]:The full-length cDNA sequence of the LiNramp2 gene of Iris Louisiana was cloned by RACE technique. Its size was 1 786 BP, which was predicted to encode 519 amino acids. The protein had 11 transmembrane domains and had more than 80% homology with other Nramp proteins.These results suggest that LiNramp2 is highly conserved with other homologous genes.The quantitative expression of LiNramp2 in leaves and stamens of Iris Louisiana was higher than that in pistil, but there was no difference between root and stem.With the increase of lead treatment time, the expression of LiNramp2 gene decreased slowly in the root system, but increased first and then decreased in the leaves, but the difference was not significant.
【作者单位】：苏州农业职业技术学院;江苏省·中国科学院植物研究所(南京中山植物园);南京农业大学;
【基金】：江苏省教育厅‘青蓝工程’科技创新团队项目(No.2014-24);江苏省教育厅‘青蓝工程’中青年学术带头人项目(No.2012-246) 苏州市科技支撑计划项目(No.SNG201209)
【分类号】：S682.19

【共引文献】